Invitrogen

Pyruvate Carboxylase Polyclonal Antibody

View all (13) Pyruvate Carboxylase antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (4)

Pyruvate Carboxylase Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

Pyruvate Carboxylase Antibody (PA5-101799) in ICC/IF

Immunofluorescent analysis of Pyruvate Carboxylase in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Pyruvate Carboxylase polyclonal antibody (Product # PA5-101799) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mou... View More

Product Image Gallery

Pyruvate Carboxylase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Pyruvate Carboxylase in human lung cancer tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Pyruvate Carboxylase polyclonal antibody (Product # PA5-101799) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Pyruvate Carboxylase Antibody in Western Blot (WB) — Western blot analysis of Pyruvate Carboxylase in PC12 cell lysate (left lane: treated with blocking peptide). Samples were incubated with Pyruvate Carboxylase polyclonal antibody (Product # PA5-101799).

Product Details

PA5-101799

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PC(Accession P11498), corresponding to amino acid residues N370-T391.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2851231

Target Information

Pyruvate Carboxylase requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.