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Product Details
NE146-7S
| Applications | Tested Dilution | Publications |
|---|---|---|
Immunocytochemistry (ICC/IF) |
Assay-dependent | - |
ELISA (ELISA) |
Assay-dependent | - |
Dot blot (DB) |
Assay-dependent | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Rabbit |
Host/Isotype |
Sheep / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Rabbit Pyruvate kinase |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Concentration |
10 mg/mL |
Purification |
Protein G |
Storage buffer |
PBS |
Contains |
no preservative |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
Product Specific Information
Reconstituted by adding 1.0 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Target Information
PKM2 is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. PKM2 has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP:pyruvate 2-o-phosphotransferase; isozymes M1/M2; m2pk; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; pyruvate kinase, muscle; Threonine-protein kinase PKM2; tu; Tyrosine-protein kinase PKM2
Gene Aliases: PKM; PKM2
UniProt ID: (Rabbit) P11974
Entrez Gene ID: (Rabbit) 100008676
Molecular Function:
kinase
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