|Tested species reactivity||Human, Non-human primate|
|Published species reactivity||Sea urchin, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) G T R D D E Y D Y L F K V V L I C(17) of human Rab11.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||6.6 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-775 detects Rab 11 from human and non-human primate samples. This antibody detects both A and B isoforms.
PA1-775 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~22 kDa protein representing Rab 11 in CV1, PC3, and HeLa cell extract. Another less prominent, unknown band is also detected by this antibody at ~35 kDa.
The PA1-775 immunizing peptide corresponds to amino acid residues 1-17 from human Rab 11. This sequence is completely conserved in human, mouse and rat and 94% conserved in C. elegans and amphibians. This peptide (Cat. # PEP-195) is available for use in neutralization and control experiments.
Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 Rab proteins that have been described in mammals. Rab 11 is a known marker for protein trafficking, sorting, and recycling in the endosomal pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Actin polymerization controls the activation of multidrug efflux at fertilization by translocation and fine-scale positioning of ABCB1 on microvilli.
PA1-775 was used in immunohistochemistry to study the role of actin polymerization in controling ABCB1a multidrug efflux activation at fertilization
|Whalen K,Reitzel AM,Hamdoun A||Molecular biology of the cell (23:3663)||2012|
Identification of compartments involved in mammalian subcellular trafficking pathways by indirect immunofluorescence.
PA1-775 was used in immunocytochemistry to evaluate methods for the compartment identification in subcellular trafficking
|Doody A,Putnam D||Methods in molecular medicine (127:127)||2006|