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RAB27A Polyclonal Antibody

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RAB27A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using RAB27A antibody 17817-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

RAB27A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human stomach tissue slide using 17817-1-AP ( RAB27A Antibody) at dilution of 1:200 (under 40x lens).

RAB27A Antibody in Western Blot (WB) — Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 17817-1-AP (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

RAB27A Antibody in Immunoprecipitation (IP) — IP result of anti-RAB27A (IP:17817-1-AP, 4ug; Detection:17817-1-AP 1:1000) with K-562 cells lysate 2760 ug.

Product Details

17817-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 18 publications 18 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 3 publications 3 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Chicken, Dog, Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAB27A Fusion Protein Ag12136 (1-221 aa encoded by BC107680) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MSDGDYDYL IKFLALGDSG VGKTSVLYQY TDGKFNSKFI TTVGIDFREK RVVYRASGPD GATGRGQRIH LQLWDTAGQE RFRSLTTAFF RDAMGFLLLF DLTNEQSFLN VRNWISQLQM HAYCENPDIV LCGNKSDLED QRVVKEEEAI ALAEKYGIPY FETSAANGTN ISQAIEMLLD LIMKRMERCV DKSWIPEGVV RSNGHASTDQ LSEEKEKGAC GC (1-221 aa encoded by BC107680)

Target Information

The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Citrate reduced oxidative damage in stem cells by regulating cellular redox signaling pathways and represent a potential treatment for oxidative stress-induced diseases.

Redox biology

Wu X,Dai H,Liu L,Xu C,Yin Y,Yi J,Bielec MD,Han Y,Li S

Fri Feb 01 00:00:00 EST 2019

A RAB27A 5' untranslated region structural variant associated with late-onset hemophagocytic lymphohistiocytosis and normal pigmentation.

The Journal of allergy and clinical immunology

Tesi B,Rascon J,Chiang SCC,Burnyte B,Löfstedt A,Fasth A,Heizmann M,Juozapaite S,Kiudeliene R,Kvedaraite E,Miseviciene V,Muleviciene A,Müller ML,Nordenskjöld M,Matuzeviciene R,Samaitiene R,Speckmann C,Stankeviciene S,Zekas V,Voss M,Ehl S,Vaiciene-Magistris N,Henter JI,Meeths M,Bryceson YT

Sun Jul 01 00:00:00 EDT 2018

High-throughput screening identified selective inhibitors of exosome biogenesis and secretion: A drug repurposing strategy for advanced cancer.

Scientific reports

Datta A,Kim H,McGee L,Johnson AE,Talwar S,Marugan J,Southall N,Hu X,Lal M,Mondal D,Ferrer M,Abdel-Mageed AB

Fri May 25 00:00:00 EDT 2018

Zoledronate modulates intracellular vesicle trafficking in mast cells via disturbing the interaction of myosinVa/Rab3a and sytaxin4/VAMP7.

Biochemical pharmacology

Liu S,Sahid MNA,Takemasa E,Maeyama K,Mogi M

Tue May 01 00:00:00 EDT 2018

Essential Role of the a3 Isoform of V-ATPase in Secretory Lysosome Trafficking via Rab7 Recruitment.

Scientific reports

Matsumoto N,Sekiya M,Tohyama K,Ishiyama-Matsuura E,Sun-Wada GH,Wada Y,Futai M,Nakanishi-Matsui M

Mon Apr 30 00:00:00 EDT 2018

Essential Role of the a3 Isoform of V-ATPase in Secretory Lysosome Trafficking via Rab7 Recruitment.

Scientific reports

Matsumoto N,Sekiya M,Tohyama K,Ishiyama-Matsuura E,Sun-Wada GH,Wada Y,Futai M,Nakanishi-Matsui M

Mon Apr 30 00:00:00 EDT 2018

Manumycin A suppresses exosome biogenesis and secretion via targeted inhibition of Ras/Raf/ERK1/2 signaling and hnRNP H1 in castration-resistant prostate cancer cells.

Cancer letters

Datta A,Kim H,Lal M,McGee L,Johnson A,Moustafa AA,Jones JC,Mondal D,Ferrer M,Abdel-Mageed AB

Wed Nov 01 00:00:00 EDT 2017

Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Gomi H,Osawa H,Uno R,Yasui T,Hosaka M,Torii S,Tsukise A

Wed Nov 01 00:00:00 EDT 2017

Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Gomi H,Osawa H,Uno R,Yasui T,Hosaka M,Torii S,Tsukise A

Wed Nov 01 00:00:00 EDT 2017

Rab27A overexpression promotes bladder cancer proliferation and chemoresistance through regulation of NF-κB signaling.

Oncotarget

Liu J,Gong X,Zhu X,Xue D,Liu Y,Wang P

Tue Sep 26 00:00:00 EDT 2017

Rab27A overexpression promotes bladder cancer proliferation and chemoresistance through regulation of NF-κB signaling.

Oncotarget

Liu J,Gong X,Zhu X,Xue D,Liu Y,Wang P

Tue Sep 26 00:00:00 EDT 2017

Exosome-mediated miR-146a transfer suppresses type I interferon response and facilitates EV71 infection.

PLoS pathogens

Fu Y,Zhang L,Zhang F,Tang T,Zhou Q,Feng C,Jin Y,Wu Z

Fri Sep 01 00:00:00 EDT 2017

Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-γ-stimulated lung epithelial cells.

Scientific reports

Chen YD,Fang YT,Cheng YL,Lin CF,Hsu LJ,Wang SY,Anderson R,Chang CP,Lin YS

Tue Jul 18 00:00:00 EDT 2017

Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-γ-stimulated lung epithelial cells.

Scientific reports

Chen YD,Fang YT,Cheng YL,Lin CF,Hsu LJ,Wang SY,Anderson R,Chang CP,Lin YS

Tue Jul 18 00:00:00 EDT 2017

Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.

Nature communications

Takahashi A,Okada R,Nagao K,Kawamata Y,Hanyu A,Yoshimoto S,Takasugi M,Watanabe S,Kanemaki MT,Obuse C,Hara E

Tue May 16 00:00:00 EDT 2017

Autophagy is dispensable for Kmt2a/Mll-Mllt3/Af9 AML maintenance and anti-leukemic effect of chloroquine.

Autophagy

Chen X,Clark J,Wunderlich M,Fan C,Davis A,Chen S,Guan JL,Mulloy JC,Kumar A,Zheng Y

Thu May 04 00:00:00 EDT 2017

Extracellular vesicles released by CD40/IL-4-stimulated CLL cells confer altered functional properties to CD4+ T cells.

Blood

Smallwood DT,Apollonio B,Willimott S,Lezina L,Alharthi A,Ambrose AR,De Rossi G,Ramsay AG,Wagner SD

Thu Jul 28 00:00:00 EDT 2016

Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation.

Histochemistry and cell biology

Wang HH,Cui Q,Zhang T,Wang ZB,Ouyang YC,Shen W,Ma JY,Schatten H,Sun QY

Wed Jun 01 00:00:00 EDT 2016

Expression of Secretogranin III in Chicken Endocrine Cells: Its Relevance to the Secretory Granule Properties of Peptide Prohormone Processing and Bioactive Amine Content.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Gomi H,Morikawa S,Shinmura N,Moki H,Yasui T,Tsukise A,Torii S,Watanabe T,Maeda Y,Hosaka M

Fri May 01 00:00:00 EDT 2015

Phospholipase C-related catalytically inactive protein (PRIP) controls KIF5B-mediated insulin secretion.

Biology open

Asano S,Nemoto T,Kitayama T,Harada K,Zhang J,Harada K,Tanida I,Hirata M,Kanematsu T

Thu May 08 00:00:00 EDT 2014

Unexpected gain of function for the scaffolding protein plectin due to mislocalization in pancreatic cancer.

Proceedings of the National Academy of Sciences of the United States of America

Shin SJ,Smith JA,Rezniczek GA,Pan S,Chen R,Brentnall TA,Wiche G,Kelly KA

Tue Nov 26 00:00:00 EST 2013

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Nature immunology

Li J,Liu K,Liu Y,Xu Y,Zhang F,Yang H,Liu J,Pan T,Chen J,Wu M,Zhou X,Yuan Z

Thu Aug 01 00:00:00 EDT 2013

Golgi fragmentation is Rab and SNARE dependent in cellular models of Parkinson's disease.

Histochemistry and cell biology

Rendón WO,Martínez-Alonso E,Tomás M,Martínez-Martínez N,Martínez-Menárguez JA

Wed May 01 00:00:00 EDT 2013

Cdx2 regulates endo-lysosomal function and epithelial cell polarity.

Genes & development

Gao N,Kaestner KH

Tue Jun 15 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: GTP-binding protein Ram; mutant Ras-related protein Rab-27A; Rab-27; Ras-related protein Rab-27A

Gene Aliases: GS2; HsT18676; RAB27; RAB27A; RAM

UniProt ID: (Human) P51159

Entrez Gene ID: (Human) 5873

Function(s)

protein targeting exocytosis small GTPase mediated signal transduction blood coagulation positive regulation of gene expression antigen processing and presentation melanocyte differentiation melanosome localization melanosome transport multivesicular body organization cytotoxic T cell degranulation natural killer cell degranulation positive regulation of exocytosis synaptic vesicle transport positive regulation of phagocytosis multivesicular body sorting pathway complement-dependent cytotoxicity positive regulation of regulated secretory pathway positive regulation of reactive oxygen species biosynthetic process positive regulation of constitutive secretory pathway exosomal secretion

Process(es)

GTPase activity protein binding GTP binding GDP binding protein domain specific binding myosin V binding

RAB27A Polyclonal Antibody

Product Details

17817-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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