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RAB35 Polyclonal Antibody

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RAB35 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HeLa cells using 11329-2-AP (RAB35 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

RAB35 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11329-2-AP ( RAB35 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

RAB35 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 11329-2-AP (RAB35 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

RAB35 Antibody in Immunoprecipitation (IP) — IP result of anti-RAB35 (IP:11329-2-AP, 4 ug; Detection:11329-2-AP 1:600) with mouse brain tissue lysate 2400 ug.

Product Details

11329-2-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 19 publications 19 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 7 publications 7 publications
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAB35 Fusion Protein Ag1872 (1-201 aa encoded by BC015931) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MARDYDHLF KLLIIGDSGV GKSSLLLRFA DNTFSGSYIT TIGVDFKIRT VEINGEKVKL QIWDTAGQER FRTITSTYYR GTHGVIVVYD VTSAESFVNV KRWLHEINQN CDDVCRILVG NKNDDPERKV VETEDAYKFA GQMGIQLFET SAKENVNVEE MFNCITELVL RAKKDNLAKQ QQQQQNDVVK LTKNSKRKKR CC (1-201 aa encoded by BC015931)

Target Information

RAB35 possesses GTPase activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NDP52 interacts with mitochondrial RNA poly(A) polymerase to promote mitophagy.

EMBO reports

Furuya N,Kakuta S,Sumiyoshi K,Ando M,Nonaka R,Suzuki A,Kazuno S,Saiki S,Hattori N

Sat Dec 01 00:00:00 EST 2018

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Nature communications

Corallino S,Malinverno C,Neumann B,Tischer C,Palamidessi A,Frittoli E,Panagiotakopoulou M,Disanza A,Malet-Engra G,Nastaly P,Galli C,Luise C,Bertalot G,Pece S,Di Fiore PP,Gauthier N,Ferrari A,Maiuri P,Scita G

Mon Apr 16 00:00:00 EDT 2018

A RAB35-p85/PI3K axis controls oscillatory apical protrusions required for efficient chemotactic migration.

Nature communications

Mon Apr 16 00:00:00 EDT 2018

Dysregulated phosphorylation of Rab GTPases by LRRK2 induces neurodegeneration.

Molecular neurodegeneration

Jeong GR,Jang EH,Bae JR,Jun S,Kang HC,Park CH,Shin JH,Yamamoto Y,Tanaka-Yamamoto K,Dawson VL,Dawson TM,Hur EM,Lee BD

Tue Feb 13 00:00:00 EST 2018

Rab35 GTPase recruits NDP52 to autophagy targets.

The EMBO journal

Minowa-Nozawa A,Nozawa T,Okamoto-Furuta K,Kohda H,Nakagawa I

Fri Sep 15 00:00:00 EDT 2017

Rab35 GTPase recruits NDP52 to autophagy targets.

The EMBO journal

Minowa-Nozawa A,Nozawa T,Okamoto-Furuta K,Kohda H,Nakagawa I

Fri Sep 15 00:00:00 EDT 2017

Rab35 protein regulates evoked exocytosis of endothelial Weibel-Palade bodies.

The Journal of biological chemistry

Biesemann A,Gorontzi A,Barr F,Gerke V

Fri Jul 14 00:00:00 EDT 2017

Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2.

Nature communications

Takasugi M,Okada R,Takahashi A,Virya Chen D,Watanabe S,Hara E

Tue Jun 06 00:00:00 EDT 2017

Increased Rab35 expression is a potential biomarker and implicated in the pathogenesis of Parkinson's disease.

Oncotarget

Chiu CC,Yeh TH,Lai SC,Weng YH,Huang YC,Cheng YC,Chen RS,Huang YZ,Hung J,Chen CC,Lin WY,Chang HC,Chen YJ,Chen CL,Chen HY,Lin YW,Wu-Chou YH,Wang HL,Lu CS

Tue Aug 23 00:00:00 EDT 2016

Increased Rab35 expression is a potential biomarker and implicated in the pathogenesis of Parkinson's disease.

Oncotarget

Chiu CC,Yeh TH,Lai SC,Weng YH,Huang YC,Cheng YC,Chen RS,Huang YZ,Hung J,Chen CC,Lin WY,Chang HC,Chen YJ,Chen CL,Chen HY,Lin YW,Wu-Chou YH,Wang HL,Lu CS

Tue Aug 23 00:00:00 EDT 2016

Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sheehan P,Zhu M,Beskow A,Vollmer C,Waites CL

Wed Aug 17 00:00:00 EDT 2016

Activity-Dependent Degradation of Synaptic Vesicle Proteins Requires Rab35 and the ESCRT Pathway.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sheehan P,Zhu M,Beskow A,Vollmer C,Waites CL

Wed Aug 17 00:00:00 EDT 2016

Rab3A, Rab27A, and Rab35 regulate different events during mouse oocyte meiotic maturation and activation.

Histochemistry and cell biology

Wang HH,Cui Q,Zhang T,Wang ZB,Ouyang YC,Shen W,Ma JY,Schatten H,Sun QY

Wed Jun 01 00:00:00 EDT 2016

Identification of an oncogenic RAB protein.

Science (New York, N.Y.)

Wheeler DB,Zoncu R,Root DE,Sabatini DM,Sawyers CL

Fri Oct 09 00:00:00 EDT 2015

Sorting of Clathrin-Independent Cargo Proteins Depends on Rab35 Delivered by Clathrin-Mediated Endocytosis.

Traffic (Copenhagen, Denmark)

Dutta D,Donaldson JG

Tue Sep 01 00:00:00 EDT 2015

Novel functions for the endocytic regulatory proteins MICAL-L1 and EHD1 in mitosis.

Traffic (Copenhagen, Denmark)

Reinecke JB,Katafiasz D,Naslavsky N,Caplan S

Thu Jan 01 00:00:00 EST 2015

CLIC4 regulates cell adhesion and β1 integrin trafficking.

Journal of cell science

Argenzio E,Margadant C,Leyton-Puig D,Janssen H,Jalink K,Sonnenberg A,Moolenaar WH

Mon Dec 15 00:00:00 EST 2014

Rab35, acting through ACAP2 switching off Arf6, negatively regulates oligodendrocyte differentiation and myelination.

Molecular biology of the cell

Miyamoto Y,Yamamori N,Torii T,Tanoue A,Yamauchi J

Thu May 01 00:00:00 EDT 2014

Syntaxin 16 is a master recruitment factor for cytokinesis.

Molecular biology of the cell

Neto H,Kaupisch A,Collins LL,Gould GW

Sun Dec 01 00:00:00 EST 2013

Neurotransmitter-triggered transfer of exosomes mediates oligodendrocyte-neuron communication.

PLoS biology

Frühbeis C,Fröhlich D,Kuo WP,Amphornrat J,Thilemann S,Saab AS,Kirchhoff F,Möbius W,Goebbels S,Nave KA,Schneider A,Simons M,Klugmann M,Trotter J,Krämer-Albers EM

Mon Jul 01 00:00:00 EDT 2013

Rab35 is required for Wnt5a/Dvl2-induced Rac1 activation and cell migration in MCF-7 breast cancer cells.

Cellular signalling

Zhu Y,Shen T,Liu J,Zheng J,Zhang Y,Xu R,Sun C,Du J,Chen Y,Gu L

Wed May 01 00:00:00 EDT 2013

Rab35 is required for Wnt5a/Dvl2-induced Rac1 activation and cell migration in MCF-7 breast cancer cells.

Cellular signalling

Zhu Y,Shen T,Liu J,Zheng J,Zhang Y,Xu R,Sun C,Du J,Chen Y,Gu L

Wed May 01 00:00:00 EDT 2013

Rab35 is required for Wnt5a/Dvl2-induced Rac1 activation and cell migration in MCF-7 breast cancer cells.

Cellular signalling

Zhu Y,Shen T,Liu J,Zheng J,Zhang Y,Xu R,Sun C,Du J,Chen Y,Gu L

Wed May 01 00:00:00 EDT 2013

TBC1D13 is a RAB35 specific GAP that plays an important role in GLUT4 trafficking in adipocytes.

Traffic (Copenhagen, Denmark)

Davey JR,Humphrey SJ,Junutula JR,Mishra AK,Lambright DG,James DE,Stöckli J

Mon Oct 01 00:00:00 EDT 2012

MICAL-L1 is a tubular endosomal membrane hub that connects Rab35 and Arf6 with Rab8a.

Traffic (Copenhagen, Denmark)

Rahajeng J,Giridharan SS,Cai B,Naslavsky N,Caplan S

Sun Jan 01 00:00:00 EST 2012

Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35.

Cell structure and function

Fukuda M,Kobayashi H,Ishibashi K,Ohbayashi N

Fri Dec 30 00:00:00 EST 2011

Rab35 regulates phagosome formation through recruitment of ACAP2 in macrophages during FcγR-mediated phagocytosis.

Journal of cell science

Egami Y,Fukuda M,Araki N

Tue Nov 01 00:00:00 EDT 2011

EPI64 protein functions as a physiological GTPase-activating protein for Rab27 protein and regulates amylase release in rat parotid acinar cells.

The Journal of biological chemistry

Imai A,Yoshie S,Ishibashi K,Haga-Tsujimura M,Nashida T,Shimomura H,Fukuda M

Fri Sep 30 00:00:00 EDT 2011

British journal of cancer

Sheach LA,Adeney EM,Kucukmetin A,Wilkinson SJ,Fisher AD,Elattar A,Robson CN,Edmondson RJ

Tue Aug 04 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: GTP-binding protein RAY; Ras-related protein Rab-1C; ras-related protein rab-1c (GTP-binding protein ray); Ras-related protein Rab-35

Gene Aliases: 9530019H02Rik; AU040256; H-ray; RAB1C; RAB35; RAY

UniProt ID: (Human) Q15286, (Mouse) Q6PHN9, (Rat) Q5U316

Entrez Gene ID: (Human) 11021, (Mouse) 77407, (Rat) 288700

Function(s)

cytokinesis ER to Golgi vesicle-mediated transport small GTPase mediated signal transduction protein localization protein transport endosomal transport antigen processing and presentation neuron projection development endocytic recycling protein localization to endosome plasma membrane to endosome transport cellular response to nerve growth factor stimulus transport intracellular protein transport metabolic process Rab protein signal transduction

Process(es)

GTPase activity protein binding GTP binding phosphatidylinositol-4,5-bisphosphate binding GDP binding nucleotide binding

RAB35 Polyclonal Antibody

Product Details

11329-2-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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