Invitrogen

RAB3A Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

2 References

View all (23) RAB3a antibodies

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Datasheet

Protocols

Questions & Answers

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RAB3A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of RAB3A was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RAB3A Rabbit Polyclonal Antibody (Product # PA1-770) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing Cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

RAB3A Antibody in Western Blot (WB) — Western blot was performed using Anti-RAB3A Polyclonal Antibody (Product # PA1-770) and a 27 kDa band corresponding to RAB3A was observed along with two uncharacterized band (*) at ~40 kD and 60 kDa across tissues tested. Tissue extract (30 µg lysate) of Mouse Brain (Lane 1), Mouse Kidney (Lane 2), Mouse Liver (Lane 3), Rat Brain (Lane 4), Rat Kidney (Lane 5), Rat Liver (Lane 6), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

RAB3A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Relative expression of RAB3A was observed in Mouse Brain and Rat Brain in comparison to Mouse Kidney, Mouse Liver, Rat Kidney and Rat Liver using Anti-RAB3A Polyclonal Antibody (Product # PA1-770) in Western Blot. {RE}

Product Details

PA1-770

Applications	Tested Dilution	Publications
Western Blot (WB)	2 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	2 µg/mL	-
Immunoprecipitation (IP)	Assay-dependent	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Dog, Hamster, Human, Mouse, Rat
Published species	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues M(1) A S A T D S R Y G Q K E S S D Q N(18) C of human RAB3A. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2177381

Product Specific Information

PA1-770 detects rab 3A from canine, hamster, human, mouse and rat tissues and cells.

PA1-770 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~21 kDa protein representing rab 3A from Hepa1 cell lysate.

PA1-770 immunogen is a synthetic peptide corresponding to residues M(1) A S A T D S R Y G Q K E S S D Q N(18) C of human RAB3A. This sequence is completely conserved between human, mouse, and rat rab 3A. PA1-770 immunizing peptide (Cat. # PEP-098) is available for use in neutralization and control experiments.

Recombinant human rab 3A (Cat # RP-770) protein can be purchased for control experiments in Western blot applications.

Target Information

Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has 4 known isoforms: 3A, 3B, 3C, and 3D. These proteins are expressed in highest concentrations in brain and endocrine tissues and involved in regulated exocytosis of hormones and neurotransmitters. Specifically, Rab 3s are thought to play a key role in presynaptic vesicle trafficking and priming.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Secreted key regulators (Fgf1, Bmp4, Gdf3) are expressed by PAC1-immunopositive retinal ganglion cells in the postnatal rat retina.

European journal of histochemistry : EJH

Dénes V,Kovacs K,Lukáts Á,Mester A,Berta G,Szabó A,Gabriel R

PA1-770 was used in Immunohistochemistry to show that these proteins could regulate postnatal RGC development acting through intracrine pathways.

Wed Apr 27 00:00:00 EDT 2022

Systematic proteomic analysis of LRRK2-mediated Rab GTPase phosphorylation establishes a connection to ciliogenesis.

eLife

Steger M,Diez F,Dhekne HS,Lis P,Nirujogi RS,Karayel O,Tonelli F,Martinez TN,Lorentzen E,Pfeffer SR,Alessi DR,Mann M

PA1-770 was used in Western Blotting to assess the LRRK2-mediated phosphorylation of the Rab GTPase family to establish a connection to primary ciliogenesis.

Fri Nov 10 00:00:00 EST 2017

Bioinformatics

Protein Aliases: C18A3.6; RAB3A, member RAS oncogene family; RAS-associated protein RAB3A; Ras-related protein Rab-3A; ras-related protein Rab-3A-like protein; Ras-related small GTP binding protein 3A

Gene Aliases: RAB3; RAB3A

UniProt ID: (Human) P20336, (Rat) P63012, (Mouse) P63011

Entrez Gene ID: (Dog) 484826, (Human) 5864, (Chinese hamster) 100759024, (Rat) 25531, (Mouse) 19339

Function(s)

respiratory system process intracellular protein transport vesicle docking involved in exocytosis mitochondrion organization neuromuscular synaptic transmission axonogenesis metabolic process protein secretion post-embryonic development synaptic vesicle exocytosis synaptic vesicle maturation regulation of exocytosis lung development regulation of synaptic vesicle fusion to presynaptic membrane Rab protein signal transduction positive regulation of ATPase activity synaptic vesicle recycling regulation of short-term neuronal synaptic plasticity maintenance of presynaptic active zone structure sensory perception of touch response to electrical stimulus protein localization to plasma membrane positive regulation of regulated secretory pathway transport exocytosis small GTPase mediated signal transduction protein transport synaptic vesicle transport evoked neurotransmitter secretion regulation of long-term synaptic potentiation neurotransmitter secretion glutamate secretion constitutive secretory pathway positive regulation of exocytosis

Process(es)

ATPase activator activity GTPase activity protein binding GTP binding protein C-terminus binding myosin V binding GTPase binding ATPase binding GDP binding nucleotide binding

RAB3A Polyclonal Antibody

Product Details

PA1-770

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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