Proteintech

RAB5B Polyclonal Antibody

View all (8) RAB5B antibodies

Questions & Answers

Questions & Answers

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RAB5B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol) fixed NIH/3T3 cells using 27403-1-AP (RAB5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

RAB5B Antibody in Western Blot (WB) — Mouse lung tissue were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RAB5B Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RAB5B Antibody in Western Blot (WB) — NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RAB5B Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RAB5B Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

RAB5B Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 27403-1-AP (RAB5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RAB5B Antibody in Western Blot (WB) — WB result of RAB5B antibody (27403-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB5B transfected HeLa cells.

Product Details

27403-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAB5B Fusion Protein Ag26584 (132-215 aa encoded by BC032740) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: GNKADLANK RMVEYEEAQA YADDNSLLFM ETSAKTAMNV NDLFLAIAKK LPKSEPQNLG GAAGRSRGVD LHEQSQQNKS QCCSN (132-215 aa encoded by BC032740)

Target Information

RAB5B play a role in protein transport. RAB5B is probably involved in vesicular traffic.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Ras-related protein Rab-5B

Gene Aliases: C030027M18Rik; RAB5B

UniProt ID: (Human) P61020, (Mouse) P61021

Entrez Gene ID: (Human) 5869, (Mouse) 19344

endosome organization small GTPase mediated signal transduction protein transport antigen processing and presentation regulation of endocytosis plasma membrane to endosome transport transport intracellular protein transport metabolic process Rab protein signal transduction

Process(es)

GTPase activity protein binding GTP binding GDP binding GTP-dependent protein binding nucleotide binding