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          • Primary Antibodies ›
          • RAC1/RAC2/RAC3 Antibodies

          Invitrogen

          RAC1/RAC2/RAC3 Recombinant Rabbit Monoclonal Antibody (2J4S6)

          View all (7) RAC1/RAC2/RAC3 antibodies

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          Cite RAC1/RAC2/RAC3 Recombinant Rabbit Monoclonal Antibody (2J4S6)

          Additional Information:
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          • Antibody Testing Data (1)
          RAC1/RAC2/RAC3 Antibody in Western Blot (WB)
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          RAC1/RAC2/RAC3 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          RAC1/RAC2/RAC3 Antibody (MA5-35636) in WB

          Western blot analysis of RAC1/RAC2/RAC3 in extracts of various cell lines. Samples were incubated with RAC1/RAC2/RAC3 Monoclonal antibody (Product # MA5-35636) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          RAC1/RAC2/RAC3 Antibody in Western Blot (WB)
          RAC1/RAC2/RAC3 Recombinant Rabbit Monoclonal Antibody (2J4S6)

          Product Details

          MA5-35636

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          2J4S6

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human RAC1(P63000)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.6 mg/mL

          Amount

          160 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol, 0.05% BSA

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2849536

          Product Specific Information

          Immunogen sequence: MQAIKCVVVG DGAVGKTCLL ISYTTNAFPG EYIPTVFDNY SANVMVDGKP VNLGLWDTAG QEDYDRLRPL SYPQTDVFLI CFSLVSPASF ENVRAKWYPE VRHHCPNTPI ILVGTKLDLR DDKDTIEKLK EKKLTPITYP QGLAMAKEIG AVKYLECSAL TQRGLKTVFD EAIRAVLCPP PVKKRKRKC

          Target Information

          The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Cell migration-inducing gene 5 protein; EN-7 protein; GX; p21-Rac1; p21-Rac2; p21-Rac3; Protein EN-7; Rac1ins; RAC2; ras-like protein; Ras-like protein TC25; RAS-related C3 botulinum substrate 2; ras-related C3 botulinum toxin substrate; Ras-related C3 botulinum toxin substrate 1; ras-related C3 botulinum toxin substrate 1 (rho family small GTP binding protein Rac1); ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1); Ras-related C3 botulinum toxin substrate 2; ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2); Ras-related C3 botulinum toxin substrate 3; ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3); Ras-related C3 botulinum toxin substrate 3 (rho family, small GTP-binding protein Rac2); rho family, small GTP binding protein Rac1; rho family, small GTP binding protein Rac3; Small G protein; unnamed protein product

          View more View less

          Gene Aliases: AI323801; AI452260; AL023026; D5Ertd559e; EN-7; Gx; HSPC022; IMD73A; IMD73B; IMD73C; MIG5; MRD48; p21-Rac1; p21-Rac2; Rac; Rac-1; RAC1; Rac1B; RAC2; RAC3; TC-25; TC25

          View more View less

          UniProt ID: (Human) P63000, (Human) P15153, (Human) P60763, (Mouse) P63001, (Rat) Q6RUV5, (Mouse) Q05144, (Mouse) P60764

          View more View less

          Entrez Gene ID: (Human) 5879, (Human) 5880, (Human) 5881, (Mouse) 19353, (Rat) 363875, (Rat) 366957, (Mouse) 19354, (Mouse) 170758, (Rat) 688319

          View more View less

          Function(s)
          nucleotide binding GTPase activity obsolete small monomeric GTPase activity protein binding GTP binding hydrolase activity enzyme binding protein kinase binding GTP-dependent protein binding thioesterase binding macromolecular complex binding Rho GDP-dissociation inhibitor binding protein kinase regulator activity calcium-dependent protein binding Rab GTPase binding histone deacetylase binding small GTPase immunoglobulin
          Process(es)
          neuron migration positive regulation of protein phosphorylation auditory receptor cell morphogenesis sphingosine-1-phosphate signaling pathway epithelial cell morphogenesis endocytosis phagocytosis, engulfment inflammatory response hyperosmotic response cytoskeleton organization actin filament organization cell adhesion cell-matrix adhesion establishment or maintenance of cell polarity enzyme linked receptor protein signaling pathway G-protein coupled receptor signaling pathway small GTPase mediated signal transduction axon guidance motor neuron axon guidance regulation of cell shape response to wounding anatomical structure morphogenesis regulation of hydrogen peroxide metabolic process regulation of lamellipodium assembly positive regulation of lamellipodium assembly positive regulation of endothelial cell migration regulation of fibroblast migration negative regulation of fibroblast migration regulation of cell-substrate adhesion positive regulation of cell-substrate adhesion regulation of neuron maturation dendrite development cell migration Rac protein signal transduction cerebral cortex radially oriented cell migration embryonic olfactory bulb interneuron precursor migration cerebral cortex GABAergic interneuron development regulation of cell morphogenesis cell projection assembly lamellipodium assembly actin cytoskeleton organization actin filament polymerization regulation of cell migration positive regulation of actin filament polymerization cortical cytoskeleton organization forebrain development positive regulation of microtubule polymerization ruffle organization response to lipopolysaccharide negative regulation of interleukin-23 production regulation of actin cytoskeleton organization substrate adhesion-dependent cell spreading positive regulation of Rho protein signal transduction intracellular signal transduction non-canonical Wnt signaling pathway positive regulation of insulin secretion involved in cellular response to glucose stimulus superoxide anion generation erythrocyte enucleation engulfment of apoptotic cell cell-cell junction organization regulation of nitric oxide biosynthetic process respiratory burst regulation of JAK-STAT cascade hepatocyte growth factor receptor signaling pathway regulation of neuronal synaptic plasticity negative regulation of receptor-mediated endocytosis anatomical structure arrangement neuron projection morphogenesis dendrite morphogenesis cell motility homeostasis of number of cells within a tissue regulation of stress fiber assembly positive regulation of stress fiber assembly localization within membrane positive regulation of focal adhesion assembly positive regulation of protein kinase B signaling synaptic transmission, GABAergic Wnt signaling pathway, planar cell polarity pathway regulation of respiratory burst cell chemotaxis positive regulation of dendritic spine development regulation of cell adhesion involved in heart morphogenesis regulation of ERK5 cascade semaphorin-plexin signaling pathway dopaminergic neuron differentiation protein localization to plasma membrane angiotensin-activated signaling pathway involved in heart process positive regulation of neutrophil chemotaxis cochlea morphogenesis ruffle assembly regulation of postsynapse assembly positive regulation of substrate adhesion-dependent cell spreading positive regulation of ruffle assembly regulation of synaptic vesicle endocytosis regulation of neutrophil migration positive regulation of bicellular tight junction assembly positive regulation of skeletal muscle acetylcholine-gated channel clustering interneuron migration midbrain dopaminergic neuron differentiation positive regulation of ovarian follicle development regulation of neuron migration chemotaxis signal transduction cell proliferation regulation of T cell proliferation regulation of mast cell degranulation bone resorption regulation of mast cell chemotaxis mast cell proliferation positive regulation of mast cell proliferation lymphocyte aggregation positive regulation of protein targeting to mitochondrion Wnt signaling pathway neuron projection development positive regulation of cell adhesion mediated by integrin neuromuscular process controlling balance neuromuscular process postsynaptic actin cytoskeleton organization mast cell chemotaxis metabolic process positive regulation of phosphatidylinositol 3-kinase activity positive regulation of DNA replication cellular response to mechanical stimulus positive regulation of cell proliferation
          It has to be done as per old AB suggested Products section.

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