Immunohistochemical analysis of RACGAP1 in paraffin-embedded human testis tissue using a RACGAP1 polyclonal antibody (Product # PA1-31510) at a dilution of 1 µg/ml. Microwaved antigen retrieval was performed using a Tris/EDTA buffer, pH 9, followed by HRP-staining.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence GRQGNFFASPMLK, from C Terminus of the protein sequence according to NP_037409|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Western Blot (WB)||0.2-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31510 detects RACGAP1 from human samples.
PA1-31510 has been successfully used in Western blot applications.
The PA1-31510 immunogen is: Peptide with sequence GRQGNFFASPMLK, from C Terminus of the protein sequence according to NP_037409
Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 .. GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.