|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 133-148 of human Rad1.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Human Rad1 is a homolog of Schizosaccharomyces pombe Rad1.
Suggested positive control: Hela whole cell lysate, 293 whole cell lysate, 293, HeLa, or NIH-3T3, 3T3 whole cell lysate.
The structure and integrity of DNA molecules can be spontaneously affected through intrinsic instability of chemical bonds in DNA or can be induced by chemical compounds and irradiation. DNA lesions are of many different types including single- and double-strand breaks, inter- and intra-strand cross-links and different kinds of base modification (3,4). DNA lesions hamper processes like transcription and replication resulting in cell cycle arrest, apoptosis and genomic instability (mutagenesis). DNA lesions have been implicated in many distinct genetically inherited diseases, in carcinogenesis, origin of genetic disorders and in aging (2-4). Rad1 is an essential component of cell cycle checkpoints activated by DNA damage and incomplete DNA replication (1). Six Rad proteins (Rad1, Rad3, Rad9, Rad17, Rad26, and Hus1) are involved in the early steps of DNA damage checkpoint response in S. pombe. Human HRAD1 gene is located on chromosome 5p13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.