Invitrogen

RAD1 Polyclonal Antibody

Product Image Gallery

RAD1 Antibody in Immunofluorescence (IF) — Immunofluorescent staining of RAD1 in human cell line U-2 OS shows positivity in vesicles and nucleus but excluded from the nucleoli. Samples were probed using a RAD1 Polyclonal Antibody (Product # PA5-52326).

RAD1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of RAD1 in human urinary bladder using a RAD1 Polyclonal Antibody (Product # PA5-52326) shows moderate nuclear positivity in urothelial cells.

RAD1 Antibody in Western Blot (WB) — Western blot analysis of RAD1 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using a RAD1 Polyclonal Antibody (Product # PA5-52326).

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

0.25-2 µg/mL

Immunofluorescence (IF)

0.25-2 µg/mL

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

0.04-0.4 µg/mL

Product Specifications

Species

Human

Expression System

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human RAD1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2646274

Product Specific Information

Immunogen sequence: GLREAFSELD MTSEVLQITM SPDKPYFRLS TFGNAGSSHL DYPKDSDLME AFHCNQTQVN RYKISLLKPS TKALVLSCKV SIRTDNRGFL SLQYMIRNED GQICFVEYYC CPDEEVPESE S

Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 91%.

Target Information

Rad1 is a component of a heterotrimeric PCNA-like complex that contains Rad9 and Hus1 proteins. This complex is involved in the 9-1-1 cell cycle checkpoing response complex that plays a major role in DNA repair, by associating with Rad17 and several components of the PCNA-loading heteropentamer replication factor C. Mutants has been shown to partially restore radiation resistance and G2-checkpoint activity. It has also been shown to possess exonuclease activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cell cycle checkpoint protein Hrad1; Cell cycle checkpoint protein RAD1; checkpoint control protein HRAD1; DNA repair exonuclease rad1 homolog; DNA repair exonuclease REC1; exonuclease homolog RAD1; hRAD1; RAD1 checkpoint clamp component; RAD1 homolog; Rad1-like DNA damage checkpoint protein

Gene Aliases: HRAD1; RAD1; REC1

UniProt ID: (Human) O60671

Entrez Gene ID: (Human) 5810

Molecular Function: DNA binding protein exodeoxyribonuclease hydrolase nuclease nucleic acid binding