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Proteintech
RAD18 Polyclonal Antibody
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Product Details
18333-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
RAD18 Fusion Protein Ag12944 (1-495 aa encoded by BC001302)
Conjugate
Form
Liquid
Concentration
0.3 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody is specific to RAD18.
Immunogen sequence: MDSLAESRW PPGLAVMKTI DDLLRCGICF EYFNIAMIIP QCSHNYCSLC IRKFLSYKTQ CPTCCVTVTE PDLKNNRILD ELVKSLNFAR NHLLQFALES PAKSPASSSS KNLAVKVYTP VASRQSLKQG SRLMDNFLIR EMSGSTSELL IKENKSKFSP QKEASPAAKT KETRSVEEIA PDPSEAKRPE PPSTSTLKQV TKVDCPVCGV NIPESHINKH LDSCLSREEK KESLRSSVHK RKPLPKTVYN LLSDRDLKKK LKEHGLSIQG NKQQLIKRHQ EFVHMYNAQC DALHPKSAAE IVQEIENIEK TRMRLEASKL NESVMVFTKD QTEKEIDEIH SKYRKKHKSE FQLLVDQARK GYKKIAGMSQ KTVTITKEDE STEKLSSVCM GQEDNMTSVT NHFSQSKLDS PEELEPDREE DSSSCIDIQE VLSSSESDSC NSSSSDIIRD LLEEEEAWEA SHKNDLQDTE ISPRQNRRTR AAESAEIEPR NKRNRN (1-495 aa encoded by B C001302)
Target Information
Human Rad18 (hRad18) is homologue of the S. pombe Rad18 (3,4). In yeast, Rad6 and Rad18 play a crucial role in post-replication repair functions in gap-filling of a daughter strand on replication of damaged DNA (2). Furthermore, Rad6-Rad18 interaction is thought to be critical at an early step during lesion bypass in yeast. In vivo, Rad18 protein forms a tight complex with Rad6 through a conserved ring-finger motif (3,4). It has been suggested that Rad18-Rad6 complex binds to the damaged sites by the single-stranded DNA binding property of Rad 18. This is followed by degradation of the stalled components of DNA replication by ubiquitin-conjugating enzyme activity of Rad6 (1). The human Rad18 maps on chromosome 3p24-25, the region which is often deleted in lung, breast, ovary, and testis cancers. The human RAD18 gene codes for a protein of 495 amino acids with a predicted molecular weight of 56 kDa. The human Rad18 is ubiquitously expressed in various human tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; mRAD18Sc; post-replication repair protein RAD18SC; postreplication repair protein hRAD18p; Postreplication repair protein RAD18; RAD18 E3 ubiquitin protein ligase; RAD18 homolog; RAD18, S. cerevisiae, homolog; RING finger protein 73; RING-type E3 ubiquitin transferase RAD18
Gene Aliases: 2810024C04Rik; RAD18; Rad18sc; RNF73
UniProt ID: (Human) Q9NS91, (Mouse) Q9QXK2
Entrez Gene ID: (Human) 56852, (Mouse) 58186, (Rat) 362412
Y-form DNA binding
damaged DNA binding
protein binding
zinc ion binding
ligase activity
polyubiquitin binding
ubiquitin protein ligase binding
protein complex binding
identical protein binding
single-stranded DNA-dependent ATPase activity
ubiquitin protein ligase activity
DNA binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
molecular_function
ubiquitin-protein ligase
protein modifying enzyme
DNA repair
postreplication repair
protein monoubiquitination
cellular response to DNA damage stimulus
response to UV
DNA damage response, detection of DNA damage
protein autoubiquitination
positive regulation of chromosome segregation
negative regulation of cell death
spermatogenesis
negative regulation of DNA recombination
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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