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Identifyn
RAD50 Polyclonal Antibody, Alexa Fluor™ 680, Identifyn™
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FIGURE: 1 / 7
RAD50 Antibody (DC-000062) in ICC/IF
Identifyn Detailed protocols can be found below under Documents & Downloads. Confocal Microscopy: HeLa cells were grown on acid-etched 1.5H cover glass to ~50% confluency, then fixed in -20°C methanol and blocked with Thermo Fisher Fish Serum Blocker (Product # 37527X3). The primary antibody, Rabbit anti-Human RAD50 Polyclonal - Identifyn SRM Alexa Fluor 680, IgG, was incubated for 1 hour at room temperature at a 1:250 dilution. Cells were washed 3 times in PBS. The second primary antibody, Alpha Tubulin Monoclonal Antibody (DM1A), was inc... View More
Product Details
DC-000062
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide Sequences between Residues 1286 - 1312, referencing Uniprot Q92878 and Gene ID 101111.
Conjugate
Excitation/Emission Max
681/704 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 10% glycerol
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Identifyn™ Primary and Secondary antibodies are extensively validated in Immunofluorescence assays by traditional as well as high resolution acquisition like Widefield SIM, Confocal, Airyscan and STORM Microscopy.
Recommended dilution for traditional microscopy:
Widefield - 1:250-1:1,000
Widefield SIM - 1:250-1:1,000
Confocal - 1:250-1:1,000
Recommended dilution for Super Resolution microscopy:
SIM (Structured Illumination Microscopy) 1:100-1:1,000
Single Molecule STORM (Stochastic Optical Reconstruction Microscopy) - 1:100-1:1,000
Detailed protocols can be found below under Documents & Downloads.
Target Information
Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA repair protein RAD50; hRAD50; hsRAD50; RAD50 2; RAD50 homolog, double strand break repair protein; RAD50 PEN; RAD50-2; RAD50-3; unnamed protein product
Gene Aliases: hRad50; NBSLD; RAD50; RAD502
UniProt ID: (Human) Q92878
Entrez Gene ID: (Human) 10111
single-stranded DNA endodeoxyribonuclease activity
nucleotide binding
DNA binding
DNA helicase activity
double-stranded telomeric DNA binding
protein binding
ATP binding
3'-5' exonuclease activity
hydrolase activity
ATPase activity
protein binding, bridging
identical protein binding
single-stranded telomeric DNA binding
protein serine/threonine kinase activator activity
metal ion binding
G-quadruplex DNA binding
regulation of mitotic recombination
telomere maintenance via recombination
telomere maintenance
double-strand break repair via homologous recombination
DNA double-strand break processing
DNA repair
double-strand break repair
DNA recombination
cellular response to DNA damage stimulus
telomere maintenance via telomerase
reciprocal meiotic recombination
telomeric 3' overhang formation
positive regulation of telomere maintenance
reciprocal DNA recombination
mitotic G2/M transition checkpoint
chromosome organization
meiotic cell cycle
R-loop processing
chromosome organization involved in meiotic cell cycle
DNA strand resection involved in replication fork processing
negative regulation of telomere capping
positive regulation of double-strand break repair
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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