Western blot analysis of Rad50 was performed by loading ~300,000 cell equivalents per lane in 1X LDS reducing sample buffer (Product #NP0007) and molecular weight ladder onto a 3-8% Tris-Acetate protein gel (Product #EA0375BOX). Proteins were transferred to a PVDF membrane at 100 mA overnight. Membrane was blocked in 5% milk/TBST for 1 hour. Rad50 was detected using a polyclonal antibody (Product # PA1-16531) diluted 1:2000 at 4°C, overnight. Membrane was then probed with anti-rabbit secondary antibody at a dilution of 1:5000. Chemiluminescent detection was performed using Pierce ECL western blot substrate (Product #32106). Data courtesy of the Antibody Data Exchange Program.
|Tested species reactivity||Hamster, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||c-terminal mRad 50 (604 amino acids).|
|Storage buffer||whole serum|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is not recommended for ICC/IF applications.
Suggested positive control: antigen standard for RAD50 (transient overexpression lysate).
Rad 50, a 153kDa protein involved in DNA double strand break repair, is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.