Proteintech

RAD51 Polyclonal Antibody

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RAD51 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis tissue slide using 14961-1-AP ( RAD51 Antibody) at dilution of 1:200 (under 40x lens).

RAD51 Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 14961-1-AP ( RAD51 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RAD51 Antibody in Immunoprecipitation (IP) — IP result of anti-RAD51 (IP:14961-1-AP, 3ug; Detection:14961-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

Product Details

14961-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 10 publications 10 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAD51 Fusion Protein Ag6823 (1-242 aa encoded by BC001459) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.23 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MAMQMQLEA NADTSVEEES FGPQPISRLE QCGINANDVK KLEEAGFHTV EAVAYAPKKE LINIKGISEA KADKILAVAE RYGLSGSDVL DNVAYARAFN TDHQTQLLYQ ASAMMVESRY ALLIVDSATA LYRTDYSGRG ELSARQMHLA RFLRMLLRLA DEFGVAVVIT NQVVAQVDGA AMFAADPKKP IGGNIIAHAS TTRLYLRKGR GETRICKIYD SPCLPEAEAM FAINADGVGD AKD (1-242 aa encoded by BC001459)

Target Information

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Downregulation of Mitochondrial Single Stranded DNA Binding Protein (SSBP1) Induces Mitochondrial Dysfunction and Increases the Radiosensitivity in Non-Small Cell Lung Cancer Cells.

Journal of Cancer

Wang Y,Hu L,Zhang X,Zhao H,Xu H,Wei Y,Jiang H,Xie C,Zhou Y,Zhou F

Thu Oct 01 00:00:00 EDT 2020

Triptolide interferes with XRCC1/PARP1-mediated DNA repair and confers sensitization of triple-negative breast cancer cells to cisplatin.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zhang Z,Sun C,Zhang L,Chi X,Ji J,Gao X,Wang Y,Zhao Z,Liu L,Cao X,Yang Y,Mao W

Tue Jan 01 00:00:00 EST 2019

IER5 is involved in DNA Double-Strand Breaks Repair in Association with PAPR1 in Hela Cells.

International journal of medical sciences

Yu XP,Wu YM,Liu Y,Tian M,Wang JD,Ding KK,Ma T,Zhou PK

Thu Jun 21 00:00:00 EDT 2018

LncRNA lnc-RI regulates homologous recombination repair of DNA double-strand breaks by stabilizing RAD51 mRNA as a competitive endogenous RNA.

Nucleic acids research

Shen L,Wang Q,Liu R,Chen Z,Zhang X,Zhou P,Wang Z

Thu Jan 25 00:00:00 EST 2018

Triptolide induces DNA breaks, activates caspase-3-dependent apoptosis and sensitizes B-cell lymphoma to poly(ADP-ribose) polymerase 1 and phosphoinositide 3-kinase inhibitors.

Oncology letters

Guan J,Zhao Q,Lv J,Zhang Z,Sun S,Mao W

Sun Oct 01 00:00:00 EDT 2017

Inhibition of Rad51 sensitizes breast cancer cells with wild-type PTEN to olaparib.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Zhao Q,Guan J,Zhang Z,Lv J,Wang Y,Liu L,Zhou Q,Mao W

Sun Oct 01 00:00:00 EDT 2017

Unpredicted Downregulation of RAD51 Suggests Genome Instability Induced by Tetrachlorobenzoquinone.

Chemical research in toxicology

Song X,Shi Q,Liu Z,Wang Y,Wang Y,Song E,Song Y

Mon Dec 19 00:00:00 EST 2016

Unpredicted Downregulation of RAD51 Suggests Genome Instability Induced by Tetrachlorobenzoquinone.

Chemical research in toxicology

Song X,Shi Q,Liu Z,Wang Y,Wang Y,Song E,Song Y

Mon Dec 19 00:00:00 EST 2016

Nuclear PTEN interferes with binding of Ku70 at double-strand breaks through post-translational poly(ADP-ribosyl)ation.

Biochimica et biophysica acta

Guan J,Zhao Q,Mao W

Thu Dec 01 00:00:00 EST 2016

5-Azacytidine enhances the radiosensitivity of CNE2 and SUNE1 cells in vitro and in vivo possibly by altering DNA methylation.

PloS one

Jiang W,Li YQ,Liu N,Sun Y,He QM,Jiang N,Xu YF,Chen L,Ma J

Thu Dec 17 00:00:00 EST 2015

Radiosensitisation of bladder cancer cells by panobinostat is modulated by Ku80 expression.

Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology

Groselj B,Kerr M,Kiltie AE

Sun Sep 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; HsRAD51; RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A

Gene Aliases: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA

UniProt ID: (Human) Q06609, (Mouse) Q08297

Entrez Gene ID: (Human) 5888, (Mouse) 19361

Molecular Function: DNA metabolism protein