Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 10 publications 10 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:500 | - |
Immunocytochemistry (ICC/IF) |
- | View 1 publication 1 publication |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Published species |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
RAD51 Fusion Protein Ag6823 (1-242 aa encoded by BC001459) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.23 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: MAMQMQLEA NADTSVEEES FGPQPISRLE QCGINANDVK KLEEAGFHTV EAVAYAPKKE LINIKGISEA KADKILAVAE RYGLSGSDVL DNVAYARAFN TDHQTQLLYQ ASAMMVESRY ALLIVDSATA LYRTDYSGRG ELSARQMHLA RFLRMLLRLA DEFGVAVVIT NQVVAQVDGA AMFAADPKKP IGGNIIAHAS TTRLYLRKGR GETRICKIYD SPCLPEAEAM FAINADGVGD AKD (1-242 aa encoded by BC001459)
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; HsRAD51; RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A
Gene Aliases: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA
UniProt ID: (Human) Q06609, (Mouse) Q08297
Entrez Gene ID: (Human) 5888, (Mouse) 19361
Molecular Function:
DNA metabolism protein
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