Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000-1:5,000 | - |
Immunocytochemistry (ICC/IF) |
1:100-1:6,000 | - |
Immunoprecipitation (IP) |
Assay-dependent | - |
Product Specifications | |
---|---|
Species Reactivity |
Chicken, Human, Mouse, Xenopus |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Purified full-length recombinant Rad51 protein. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
Conc. Not Determined |
Storage buffer |
whole serum |
Contains |
0.09% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_1960219 |
PA1-4985 detects Rad51 from human, mouse, and chicken samples.
PA1-4985 has been successfully used in Western blot, immunoprecipitation, immunocytochemistry and indirect immunofluorescence applications. By Western blot, this antibody detects a 37 kDa band representing Rad51 from Hela cells. GFP-tagging of Rad51 protein at either N- or C- terminus inactivates the function of Rad51 and therefore it cannot be used for the study of foci formation instead of the antibody.
The PA1-4985 immunogen is full-length recombinant Rap51 protein.
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; homolog to S.cerevisiae; HsRAD51; Rad51 homolog; RAD51 homolog (RecA homolog, E. coli); RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A
Gene Aliases: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA
UniProt ID: (Human) Q06609, (Chicken) P37383, (Mouse) Q08297
Entrez Gene ID: (Human) 5888, (Chicken) 396086, (Mouse) 19361
Molecular Function:
DNA metabolism protein
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