|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:2,000 - 1:10,000|
|Tested Species reactivity||Chicken, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified full-length recombinant Rap51 protein.|
|Storage buffer||whole serum|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
PA1-4985 detects Rad51 from human, mouse, and chicken samples.
PA1-4985 has been successfully used in Western blot, immunoprecipitation, immunocytochemistry and indirect immunofluorescence applications. By Western blot, this antibody detects a 37 kDa band representing Rad51 from Hela cells. GFP-tagging of Rad51 protein at either N- or C- terminus inactivates the function of Rad51 and therefore it cannot be used for the study of foci formation instead of the antibody.
The PA1-4985 immunogen is full-length recombinant Rap51 protein.
Human Rad51 protein is a functional and structural homolog of E. coli RecA protein, which plays a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands. Rad51 functionally and physically interacts with its paralogs Dmc1, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3, and also with Rad52 in recombination processes. It also interacts with oncogenes and tumor suppressors such as BRACA1, BRACA2, and P53 for the maintenance of genome stability.
Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; BRCC5; DNA repair protein RAD51 homolog 1; homolog to S.cerevisiae; HRAD51; HsRAD51; HsT16930; RAD51 homolog (RecA homolog, E. coli); RAD51 homolog A; RECA; RecA, E. coli, homolog of; RecA-like protein; recombination protein A
Gene Aliases: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA