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Invitrogen

RAD51 Polyclonal Antibody

View all (37) RAD51 antibodies
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Product Details

PA1-4985

Applications Tested Dilution Publications

Western Blot (WB)

 1:1,000-1:5,000 -

Immunocytochemistry (ICC/IF)

 1:100-1:6,000 -

Immunoprecipitation (IP)

 Assay-dependent -
Product Specifications

Species Reactivity

 Chicken, Human, Mouse, Xenopus

Host/Isotype

 Rabbit / IgG

Class

 Polyclonal

Type

 Antibody

Immunogen

Purified full-length recombinant Rad51 protein.

Conjugate

 Unconjugated

Form

 Liquid

Concentration

 Conc. Not Determined

Storage buffer

 whole serum

Contains

 0.09% sodium azide

Storage conditions

 -20°C

RRID

AB_1960219

Product Specific Information

PA1-4985 detects Rad51 from human, mouse, and chicken samples.

PA1-4985 has been successfully used in Western blot, immunoprecipitation, immunocytochemistry and indirect immunofluorescence applications. By Western blot, this antibody detects a 37 kDa band representing Rad51 from Hela cells. GFP-tagging of Rad51 protein at either N- or C- terminus inactivates the function of Rad51 and therefore it cannot be used for the study of foci formation instead of the antibody.

The PA1-4985 immunogen is full-length recombinant Rap51 protein.

Target Information

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; homolog to S.cerevisiae; HsRAD51; Rad51 homolog; RAD51 homolog (RecA homolog, E. coli); RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A

Gene Aliases: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA

UniProt ID: (Human) Q06609, (Chicken) P37383, (Mouse) Q08297

Entrez Gene ID: (Human) 5888, (Chicken) 396086, (Mouse) 19361

Molecular Function: DNA metabolism protein

Function(s)
telomere maintenance via recombination double-strand break repair via homologous recombination DNA recombinase assembly DNA synthesis involved in DNA repair strand displacement regulation of protein phosphorylation DNA unwinding involved in DNA replication DNA repair DNA recombination mitotic recombination cellular response to DNA damage stimulus meiotic nuclear division reciprocal meiotic recombination response to toxic substance response to X-ray regulation of double-strand break repair via homologous recombination telomere maintenance via telomere lengthening replication fork processing strand invasion response to drug positive regulation of DNA ligation protein homooligomerization chromosome organization involved in meiotic cell cycle cellular response to ionizing radiation cellular response to gamma radiation cellular response to hydroxyurea cellular response to cisplatin cellular response to camptothecin response to etoposide replication-born double-strand break repair via sister chromatid exchange mitotic recombination-dependent replication fork processing response to ionizing radiation interstrand cross-link repair meiotic cell cycle DNA metabolic process telomere organization cellular response to alkaloid
Process(es)
recombinase activity four-way junction DNA binding chromatin binding double-stranded DNA binding single-stranded DNA binding endodeoxyribonuclease activity protein binding ATP binding protein C-terminus binding identical protein binding single-stranded DNA-dependent ATPase activity DNA polymerase binding DNA-dependent ATPase activity single-stranded DNA-dependent ATP-dependent DNA helicase activity nucleotide binding DNA binding damaged DNA binding

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