Invitrogen

RAD51 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

3 Published Figures

8 References

View all (36) RAD51 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

RAD51 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of RAD51 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RAD51 Polyclonal Antibody (Product # PA5-27195) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

RAD51 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of RAD51 was performed in paraffin-embedded human cervical carcinoma tissue using RAD51 Polyclonal Antibody (Product # PA5-27195) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

RAD51 Antibody in Western Blot (WB) — Western Blot analysis of RAD51 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RAD51 Polyclonal Antibody (Product # PA5-27195) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

RAD51 Antibody in Immunoprecipitation (IP) — PA5-27195 immunoprecipitates Rad51 protein in IP experiments. IP samples: Jurkat whole cell extract A. 40 µg Jurkat whole cell extract B. Control with 4 µg of preimmune Rabbit IgG C. Immunoprecipitation of Rad51 protein by 4 µg Rad51 antibody (Product # PA5-27195) on 5% SDS-PAGE.

RAD51 Antibody — Fig. 8 MEILB2 and BRME1 impair mitotic HR. a Mouse B16-F1 cells transfected with MEILB2 -MYC (WT) or MEILB2-R204T -MYC (R204T) stained with RAD51 and MYC antibodies. Graph: the number of MYC foci colocalizing with RAD51. n shows the analyzed RAD51-positive S to G2 phase cells. Bars: mean values with SD. b IPs with the MYC antibody from B16-F1 cells expressing MEILB2 -MYC or MEILB2-R204T -MYC with GFP- BRCA2 truncations; C (a.a. 2036-3329) or MBD (a.a. 2117-2339). c Immunostaining of zygotene spermatocytes expressing GFP- MEILB2 or GFP- MEILB2-R204T. d Mouse B16-F1 cells transfected with FLAG- BRME1 (left), MEILB2 -MYC (middle), or both FLAG- BRME1 and MEILB2 -MYC (right) and stained with RAD51 and FLAG (for BRME1) or MYC (for MEILB2) antibodies. e Mouse B16-F1 cells with or without MMC treatment transfected with MEILB2 -MYC (WT or R204T) or FLAG- BRME1 and stained with RAD51 or MYC (for MEILB2) and FLAG (for BRME1) antibodies and DAPI. Graph: the number of RAD51 foci per cell. Red bars: mean values. n shows the analyzed cell number pooled from three independent transfections. f Schematic of the U2OS-DSB reporter cell line. g U2OS-DSB reporter cell line with or without DSB induction transfected with MEILB2 -MYC or FLAG- BRME1 and stained with the RAD51 antibody. Graph: the frequency of cells with RAD51 accumulation at the DSB. The analyzed cell numbers are 78, 70, and 68 cells for mock, MEILB2, and BRME1, respectively. The mean values of two independent experiments are shown.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

View 4 publications 4 publications

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat, Zebrafish

Published species

Human, Mouse, Yeast

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 228 of Human Rad51

Conjugate

Unconjugated

Form

Liquid

Concentration

1.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2544671

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat-2.

Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (90%), Xenopus laevis (96%), Dog (98%), Pig (99%), Rabbit (97%), Chicken (95%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recobination. It binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to a single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Mutations in the gene can results in breast cancer, mirror movements 2 and fanconi anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: BRCA1/BRCA2-containing complex, subunit 5; DNA repair protein RAD51 homolog 1; HsRAD51; RAD51 homolog; RAD51 homolog (RecA homolog, E. coli); RAD51 homolog A; RecA, E. coli, homolog of; RecA-like protein; recombination protein A

Gene Aliases: AV304093; BRCC5; FANCR; HRAD51; HsRad51; HsT16930; MRMV2; RAD51; RAD51A; RECA; RGD1563603; wu:fb38e12; zgc:77754

UniProt ID: (Human) Q06609, (Mouse) Q08297

Entrez Gene ID: (Human) 5888, (Mouse) 19361, (Rat) 499870, (Zebrafish) 406487

Molecular Function: DNA metabolism protein