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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, conjugated to BSA, corresponding to residues T(254) H Q R K L R Q K Q L Q Q Q F R E R(271) of human RAD52.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2, with 50% Ammonium sulfate|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-25416 detects RAD52 from human, mouse, and rat samples. PA1-25416 is expected to cross react with chicken (94% conserved) due to sequence homology.
PA1-25416 has been successfully used in Western blot procedures.
The PA1-25416 immunogen is a synthetic peptide, conjugated to BSA, corresponding to residues T(254) H Q R K L R Q K Q L Q Q Q F R E R(271) of human RAD52.
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
DNA repair protein RAD52 homolog; recombination protein RAD52; rhabdomyosarcoma antigen MU-RMS-40.23