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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:100 | - |
Immunocytochemistry (ICC/IF) |
1:500 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Fusion protein of full length human Rad 9 protein. |
Conjugate |
Unconjugated |
Form |
Liquid |
Purification |
Protein G |
Storage buffer |
tris glycine with 0.15M NaCl |
Contains |
0.1% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_568704 |
In Western blot, a band is seen at approximately 42 kDa, however multiple bands may be seen between ~40-60 kDa, depending on phosphorylation of the protein.
Suggested positive control: antigen standard for RAD9A (transient overexpression lysate), Osteocarcinoma (U2OS) cell lysate.
RAD9 is a component of the 9-1-1 cell cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesions upon damage by the RAD17-replication factor C clamp loader complex. RAD9 acts as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD9; RAD9 homolog A
Gene Aliases: RAD9; RAD9A
UniProt ID: (Human) Q99638
Entrez Gene ID: (Human) 5883
Molecular Function:
exodeoxyribonuclease
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