In Western blot, a band is seen at approximately 42 kDa, however multiple bands may be seen between ~40-60 kDa, depending on phosphorylation of the protein.
Suggested positive control: antigen standard for RAD9A (transient overexpression lysate), Osteocarcinoma (U2OS) cell lysate.
RAD9 is a component of the 9-1-1 cell cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesions upon damage by the RAD17-replication factor C clamp loader complex. RAD9 acts as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair.
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Protein Aliases: Cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD9; RAD9 homolog A
Gene Aliases: RAD9; RAD9A
UniProt ID: (Human) Q99638
Entrez Gene ID: (Human) 5883