|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein of full length human Rad 9 protein.|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot, a band is seen at approximately 42 kDa, however multiple bands may be seen between ~40-60 kDa, depending on phosphorylation of the protein.
Suggested positive control: antigen standard for RAD9A (transient overexpression lysate), Osteocarcinoma (U2OS) cell lysate.
Human Rad 9 is a homologue of Schizosaccharomyces pombe Rad 9 protein and plays a critical role in cell cycle checkpoint control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.