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Invitrogen

RAG1 Polyclonal Antibody

View all (5) RAG1 antibodies
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Product Details

PA5-88678

Applications Tested Dilution Publications

Western Blot (WB)

 1:1,000-1:3,000 -
Product Specifications

Species Reactivity

 Human, Mouse

Host/Isotype

 Rabbit / IgG

Class

 Polyclonal

Type

 Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human RAG1

Conjugate

 Unconjugated

Form

 Liquid

Concentration

 1.62 mg/mL

Purification

 Antigen affinity chromatography

Storage buffer

 PBS, pH 7.3, with 50% glycerol

Contains

 0.02% sodium azide

Storage conditions

 -20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2805070

Product Specific Information

Immunogen sequence: MAASFPPTLG LSSAPDEIQH PHIKFSEWKF KLFRVRSFEK TPEEAQKEKK DSFEGKPSLE QSPAVLDKAD GQKPVPTQPL LKAHPKFSKK FHDNEKARGK AIHQANLRHL CRICGNSFRA DEHNRRYPVH GPVDGKTLGL LRKKEKRATS WPDLIAKVFR IDVKADVDSI HPTEFCHNCW SIMHRKFSSA PCEVYFPRNV TMEWHPHTPS CDICNTARRG LKRKSLQPNL QLSKKLKTVL DQARQARQRK RRAQARISSK DVMKKIANCS; Positive Samples: Mouse thymus; Cellular Location: Nucleus

Target Information

The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase RAG1; Endonuclease RAG1; MGC43321; RAG-1; RAG-1 Antibody; recombinase activating gene 1; recombination activating gene 1 Antibody; recombination activating protein 1; recombination-activating gene 1; RING finger protein 74; RING-type E3 ubiquitin transferase RAG1; RNF74 Antibody; V(D)J recombination-activating protein 1

Gene Aliases: RAG-1; RAG1; RNF74

UniProt ID: (Human) P15918, (Mouse) P15919

Entrez Gene ID: (Human) 5896, (Mouse) 19373

Molecular Function: DNA metabolism protein endodeoxyribonuclease nucleic acid metabolism protein

Function(s)
adaptive immune response pre-B cell allelic exclusion DNA recombination immune response histone monoubiquitination B cell differentiation T cell differentiation in thymus V(D)J recombination T cell homeostasis negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of T cell differentiation thymus development protein autoubiquitination negative regulation of thymocyte apoptotic process nucleic acid phosphodiester bond hydrolysis metabolic process obsolete death chromatin modification regulation of T cell differentiation negative regulation of T cell apoptotic process
Process(es)
DNA binding endonuclease activity ubiquitin-protein transferase activity protein binding zinc ion binding ligase activity histone binding protein homodimerization activity sequence-specific DNA binding metal ion binding ubiquitin protein ligase activity nucleic acid binding catalytic activity nuclease activity UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity hydrolase activity hydrolase activity, acting on ester bonds acid-amino acid ligase activity ribosomal S6-glutamic acid ligase activity phosphoric ester hydrolase activity T/G mismatch-specific endonuclease activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity retroviral 3' processing activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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