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Invitrogen

RAG1 Polyclonal Antibody

View all (5) RAG1 antibodies
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Product Details

Tested Applications
Dilution
Western Blot (WB)
1:1000-1:3000
Product Specifications
Species Reactivity
Mouse
Host / Isotype
Rabbit / IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human RAG1
Conjugate
Unconjugated
Form
liquid
Concentration
4.87 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen sequence: MAASFPPTLG LSSAPDEIQH PHIKFSEWKF KLFRVRSFEK TPEEAQKEKK DSFEGKPSLE QSPAVLDKAD GQKPVPTQPL LKAHPKFSKK FHDNEKARGK AIHQANLRHL CRICGNSFRA DEHNRRYPVH GPVDGKTLGL LRKKEKRATS WPDLIAKVFR IDVKADVDSI HPTEFCHNCW SIMHRKFSSA PCEVYFPRNV TMEWHPHTPS CDICNTARRG LKRKSLQPNL QLSKKLKTVL DQARQARQRK RRAQARISSK DVMKKIANCS; Positive Samples: Mouse thymus; Cellular Location: Nucleus

Target Information

The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: E3 ubiquitin-protein ligase RAG1; Endonuclease RAG1; RAG-1; recombinase activating gene 1; recombination activating protein 1; recombination-activating gene 1; RING-type E3 ubiquitin transferase RAG1; V(D)J recombination-activating protein 1

Gene Aliases: Rag-1; Rag1

UniProt ID: (Mouse) P15919

Entrez Gene ID: (Mouse) 19373

Molecular Function: DNA binding protein endodeoxyribonuclease hydrolase nuclease nucleic acid binding

Function(s)
adaptive immune response pre-B cell allelic exclusion DNA recombination metabolic process histone monoubiquitination obsolete death chromatin modification B cell differentiation T cell differentiation in thymus V(D)J recombination T cell homeostasis negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of T cell differentiation thymus development protein autoubiquitination negative regulation of T cell apoptotic process negative regulation of thymocyte apoptotic process nucleic acid phosphodiester bond hydrolysis
Process(es)
nucleic acid binding DNA binding catalytic activity nuclease activity endonuclease activity ubiquitin-protein transferase activity protein binding zinc ion binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity hydrolase activity hydrolase activity, acting on ester bonds ligase activity acid-amino acid ligase activity ribosomal S6-glutamic acid ligase activity histone binding phosphoric ester hydrolase activity protein homodimerization activity sequence-specific DNA binding T/G mismatch-specific endonuclease activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity retroviral 3' processing activity metal ion binding ubiquitin protein ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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