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RAG2 Polyclonal Antibody

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RAG2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using RAG2 antibody 11825-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

RAG2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lymphoma using 11825-1-AP (RAG2 antibody) at dilution of 1:50 (under 10x lens).

RAG2 Antibody in Western Blot (WB) — Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

RAG2 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug RAG2 antibody (11825-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

RAG2 Antibody in Immunoprecipitation (IP) — IP result of anti-RAG2 (IP:11825-1-AP, 4ug; Detection:11825-1-AP 1:300) with A375 cells lysate 3600ug.

Product Details

11825-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAG2 Fusion Protein Ag2393 (175-527 aa encoded by BC022397) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: VADCLPCVF LVDFEFGCAT SYILPELQDG LSFHVSIAKN DTIYILGGHS LANNIRPANL YRIRVDLPLG SPAVNCTVLP GGISVSSAIL TQTNNDEFVI VGGYQLENQK RMICNIISLE DNKIEIREME TPDWTPDIKH SKIWFGSNTG NGTVFLGIPG DNKQVVSEGF YFYMLKCAED DTNEEQTTFT NSQTSTEDPG DSTPFEDSEE FCFSAEANSF DGDDEFDTYN EDDEEDESET GYWITCCPTC DVDINTWVPF YSTELNKPAM IYCSHGDGHW VHAQCMDLAE RTLIHLSAGS NKYYCNEHVE IARALHTPQR VLPLKKPPMK SLRKKGSGKI LTPAKKSFLR RLFD (175-527 aa encoded by B C022397)

Target Information

RAG2 (V(D)J recombination-activating protein 2) is a core component of the RAG complex - a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity) and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: 1) first a nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends. 2) hairpin coding ends and 2 blunt, 5'-phosphorylated ends are created. The chromatin structure plays an essential rold in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and hair pinning expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin. This prevents accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. Mutations affecting the gene can results in combined cellular and humoral immune defects with granulomas, severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive, and Omenn syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Allelic exclusion of IgH through inhibition of E2A in a VDJ recombination complex.

Journal of immunology (Baltimore, Md. : 1950)

Hauser J,Grundström C,Grundström T

Sat Mar 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: RAG 2; RAG-2; recombinase activating gene 2; recombination activating protein 2; V(D)J recombination-activating protein 2

Gene Aliases: RAG-2; RAG2

UniProt ID: (Human) P55895, (Mouse) P21784

Entrez Gene ID: (Human) 5897, (Mouse) 19374

Function(s)

B cell lineage commitment pre-B cell allelic exclusion B cell homeostatic proliferation T cell lineage commitment protein ubiquitination covalent chromatin modification B cell differentiation T cell differentiation in thymus V(D)J recombination positive regulation of organ growth DNA recombination chromatin modification T cell differentiation

Process(es)

DNA binding chromatin binding phosphatidylinositol-4,5-bisphosphate binding phosphatidylinositol-3,4,5-trisphosphate binding zinc ion binding methylated histone binding phosphatidylinositol binding phosphatidylinositol-3,4-bisphosphate binding ubiquitin protein ligase activity phosphatidylinositol-3,5-bisphosphate binding protein binding metal ion binding

RAG2 Polyclonal Antibody

Product Details

11825-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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