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Immunofluorescent analysis of RAG2 in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, using a RAG2 polyclonal antibody (Product # PA5-22048). Green: Primary antibody at a dilution of 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 µm.
|Tested species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 271 and 527 of RAG2 (Uniprot ID#P55895)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-22048 targets RAG2 in ICC, IF, IHC (P), and WB applications and shows reactivity with Human and Rat samples.
The PA5-22048 immunogen is a recombinant fragment corresponding to a region within amino acids 271 and 527 of RAG2 (Uniprot ID#P55895).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
RAG-2; V(D)J recombination-activating protein 2