Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is breast tissue.
The small Ras-related protein RAN, also called TC4, is a 24kDa, nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex.
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Protein Aliases: androgen receptor-associated protein 24; ARA24; Gsp1; GTP-binding nuclear protein Ran; GTPase Ran; guanosine triphosphatase Ran; member RAS oncogene family; OK/SW-cl.81; RanGTPase; ras-like protein TC4; ras-related nuclear protein; TC4
Gene Aliases: ARA24; Gsp1; TC4