|Tested species reactivity||Bovine, Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) R R S K A D V E R Y I A S V Q G S(18) of human RanBP2.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||10 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
PA1-082 detects Ran binding protein 2 (RanBP2) from human and bovine retina.
PA1-082 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~360 kDa protein representing RanBP2 from bovine retinal extracts. Immunohistochemical staining of bovine retina with PA1-082 results in cytoplasmic staining of cells within the inner nuclear layer as well as the ganglion cells.
The PA1-082 immunogen is a synthetic peptide corresponding to residues M(1) R R S K A D V E R Y I A S V Q G S(18) of human RanBP2. This sequence is completely conserved between human and mouse. The PA1-082 immunizing peptide (Cat. # PEP-137) is available for use in neutralization and control experiments.
Transport of macromolecules into and out of the nucleus occurs through nuclear pore complexes (NPC). This energy-dependent process involves nucleoporins at the NPC, associated transport proteins and GTP. The GTPase Ran is a small protein that belongs to the RAS protein superfamily and is found associated with the nuclear membrane. Ran GTPase is essential for mRNA processing, nuclear transport, cell cycle control, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance. Ran binding proteins (RanBPs) belong to a family of proteins that bind Ran GTPase and help to stimulate its GTPase activity. Members of the RanBP family show weak similarity to importin beta, a protein involved in the transport of proteins to the nuclear membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis.
PA1-082 was used in immunocytochemistry and western blot to investigate the role of Nup358 in dynamic remodeling during muscle cell differentiation.
|Asally M,Yasuda Y,Oka M,Otsuka S,Yoshimura SH,Takeyasu K,Yoneda Y||The FEBS journal (278:610)||2011|
|Not Applicable||Not Cited||
Sumoylation delays the ATF7 transcription factor subcellular localization and inhibits its transcriptional activity.
PA1-082 was used in immunohistochemistry and western blot to study the mechanism for ATF7 protein modification by SUMO-1
|Hamard PJ,Boyer-Guittaut M,Camuzeaux B,Dujardin D,Hauss C,Oelgeschläger T,Vigneron M,Kedinger C,Chatton B||Nucleic acids research (35:1134)||2007|