Immunohistochemical analysis of RANGAP1 in paraffin-embedded human liver tissue using a RANGAP1 polyclonal antibody (Product # PA1-31482) at a dilution of 2.5 µg/ml. Steamed antigen retrieval was performed using a citrate buffer, pH 6, followed by AP-staining.
|Tested species reactivity||Bovine, Dog, Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||synthetic peptide ASEDIAKLAETLAK (Human).|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Western Blot (WB)||0.1-0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31482 detects RanGAP1 from human, mouse samples.
PA1-31482 has been successfully used in Western blot applications.
The PA1-31482 immunogen is: Synthetic peptide: ASEDIAKLAETLAK (Human).
RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1, 000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.