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RANK (soluble) Antibody (500-P144-100UG) in WB
Western Blot: To detect Human sRANK Receptor by Western Blot analysis RANK (soluble) Polyclonal Antibody (Product # 500-P144-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sRANK Receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P144-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human sRANK Receptor
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929471
Product Specific Information
AA Sequence of recombinant protein: MQIAPPCTSE KHYEHLGRCC NKCEPGKYMS SKCTTTSDSV CLPCGPDEYL DSWNEEDKCL LHKVCDTGKA LVAVVAGNST TPRRCACTAG YHWSQDCECC RRNTECAPGL GAQHPLQLNK DTVCKPCLAG YFSDAFSSTD KCRPWTNCTF LGKRVEHHGT EKSDAVCSSS LPARK.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sRANK Receptor. Anti-Human sRANK Receptor-specific antibody was purified by affinity chromatography employing an immobilized Human sRANK Receptor matrix.
Sandwich ELISA: To detect Human sRANK Receptor by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sRANK Receptor (500-P144Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sRANK Receptor.
Western Blot: To detect Human sRANK Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sRANK Receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
RANK is a member of the tumor necrosis factor receptor (TNFR) family. The full length mouse RANK cDNA encodes a type I transmembrane protein with an extracellular and a cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNFR family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD265; loss of heterozygosity, 18, chromosomal region 1; ODFR; Osteoclast differentiation factor receptor; Paget disease of bone 2; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; Tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily member 11a, NFKB activator; tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Gene Aliases: CD265; FEO; LOH18CR1; ODFR; OFE; OPTB7; OSTS; PDB2; RANK; TNFRSF11A; TRANCER
UniProt ID: (Human) Q9Y6Q6
Entrez Gene ID: (Human) 8792
ossification
adaptive immune response
monocyte chemotaxis
inflammatory response
signal transduction
cell-cell signaling
positive regulation of cell proliferation
response to radiation
osteoclast differentiation
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
response to cytokine
response to tumor necrosis factor
regulation of apoptotic process
positive regulation of JUN kinase activity
lymph node development
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of NF-kappaB transcription factor activity
circadian temperature homeostasis
mammary gland alveolus development
response to interleukin-1
positive regulation of fever generation by positive regulation of prostaglandin secretion
TNFSF11-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade via TNFSF11-mediated signaling
apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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