Proteintech

RANKL Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (8)

RANKL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

RANKL Antibody (23408-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using (Product # 23408-1-AP) (RANKL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RANKL Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

23408-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 31 publications 31 publications

Immunohistochemistry (IHC)

View 14 publications 14 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RANKL Fusion Protein Ag19975 (76-317 aa encoded by BC074890)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.28 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: PNRISEDGT HCIYRILRLH ENADFQDTTL ESQDTKLIPD SCRRIKQAFQ GAVQKELQHI VGSQHIRAEK AMVDGSWLDL AKRSKLEAQP FAHLTINATD IPSGSHKVSL SSWYHDRGWA KISNMTFSNG KLIVNQDGFY YLYANICFRH HETSGDLATE YLQLMVYVTK TSIKIPSSHT LMKGGSTKYW SGNSEFHFYS INVGGFFKLR SGEEISIEVS NPSLLDPDQD ATYFGAFKVR DID (76-317 aa encoded by BC074890)

Target Information

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cartilage Damage Pathological Characteristics of Diabetic Neuropathic Osteoarthropathy.

Analytical cellular pathology (Amsterdam)

Liu PL,Diao JY,Wang Q,Liu H,Zhang Y,Liang JQ,Zhang F,Liang XJ,Zhao HM

Fri May 19 00:00:00 EDT 2023

BML-111 inhibits osteoclast differentiation by suppressing the MAPK and NF-κB pathways, alleviating deterioration of the knee joints in a CIA rat model.

Cell biology international

Wang L,Su W,Zheng X,Lin W,Lv C,Yang S,Chen B,Zhang C

Mon May 01 00:00:00 EDT 2023

Pyrroloquinoline quinone alleviates natural aging-related osteoporosis via a novel MCM3-Keap1-Nrf2 axis-mediated stress response and Fbn1 upregulation.

Aging cell

Li J,Zhang J,Xue Q,Liu B,Qin R,Li Y,Qiu Y,Wang R,Goltzman D,Miao D,Yang R

Fri Sep 01 00:00:00 EDT 2023

Pulsed frequency modulated ultrasound promotes therapeutic effects of osteoporosis induced by ovarian failure in mice.

Ultrasonics

Zhou X,Sun S,Chen Y,Liu C,Li D,Cheng Q,He M,Li Y,Xu K,Ta D

Sat Jul 01 00:00:00 EDT 2023

Effects of green tea extract epigallocatechin-3-gallate (EGCG) on orthodontic tooth movement and root resorption in rats.

Archives of oral biology

Zou JH,Chen F,Li YL,Chen H,Sun TK,Du SM,Zhang J

Thu Jun 01 00:00:00 EDT 2023

BRG1 is involved in vascular calcification in chronic renal disease via autophagy of vascular smooth muscle cells.

iScience

Feng Y,Yu M,Wang C,Xia J,Huang L,Tang Y,Xiao Q,Pu L,Wang L,Li G,Li Y

Fri Apr 21 00:00:00 EDT 2023

Clearance of senescent cells by navitoclax (ABT263) rejuvenates UHMWPE-induced osteolysis.

International immunopharmacology

Su W,Hu Y,Fan X,Xie J

Wed Feb 01 00:00:00 EST 2023

An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1.

Experimental cell research

Hu X,Li M,Zhang Y,Sang K,Zhang Y,Li W,Liu B,Wan L,Du B,Qian J,Meng F,Fu Y,Dai M,Gao G,Ye H

Wed Mar 01 00:00:00 EST 2023

Zoledronic acid generates a spatiotemporal effect to attenuate osteoarthritis by inhibiting potential Wnt5a-associated abnormal subchondral bone resorption.

PloS one

Ding D,Wang L,Yan J,Zhou Y,Feng G,Ma L,Yang Y,Pei X,Jin Q

Mon Aug 01 00:00:00 EDT 2022

Rutin ameliorates the promotion effect of fine particulate matter on vascular calcification in calcifying vascular cells and ApoE^-/- mice.

Ecotoxicology and environmental safety

Su R,Jin X,Zhao W,Wu X,Zhai F,Li Z

Fri Apr 01 00:00:00 EDT 2022

Rutin ameliorates the promotion effect of fine particulate matter on vascular calcification in calcifying vascular cells and ApoE^-/- mice.

Ecotoxicology and environmental safety

Su R,Jin X,Zhao W,Wu X,Zhai F,Li Z

Fri Apr 01 00:00:00 EDT 2022

Rutin ameliorates the promotion effect of fine particulate matter on vascular calcification in calcifying vascular cells and ApoE^-/- mice.

Ecotoxicology and environmental safety

Su R,Jin X,Zhao W,Wu X,Zhai F,Li Z

Fri Apr 01 00:00:00 EDT 2022

Rutin ameliorates the promotion effect of fine particulate matter on vascular calcification in calcifying vascular cells and ApoE^-/- mice.

Ecotoxicology and environmental safety

Su R,Jin X,Zhao W,Wu X,Zhai F,Li Z

Fri Apr 01 00:00:00 EDT 2022

Hexokinase 2-mediated glycolysis promotes receptor activator of NF-κB ligand expression in Porphyromonas gingivalis lipopolysaccharide-treated osteoblasts.

Journal of periodontology

Yu Y,Jiang L,Li J,Lei L,Li H

Fri Jul 01 00:00:00 EDT 2022

Piezo1-mediated fluid shear stress promotes OPG and inhibits RANKL via NOTCH3 in MLO-Y4 osteocytes.

Channels (Austin, Tex.)

Liu Z,Tang Y,He L,Geng B,Lu F,He J,Yi Q,Liu X,Zhang K,Wang L,Xia Y,Jiang J

Thu Dec 01 00:00:00 EST 2022

Preconception exposure to dibutyl phthalate (DBP) impairs spermatogenesis by activating NF-κB/COX-2/RANKL signaling in Sertoli cells.

Toxicology

Xie Z,Liu S,Hua S,Wu L,Zhang Y,Zhu Y,Shi F,Jiang J

Mon May 30 00:00:00 EDT 2022

Preconception exposure to dibutyl phthalate (DBP) impairs spermatogenesis by activating NF-κB/COX-2/RANKL signaling in Sertoli cells.

Toxicology

Xie Z,Liu S,Hua S,Wu L,Zhang Y,Zhu Y,Shi F,Jiang J

Mon May 30 00:00:00 EDT 2022

Preconception exposure to dibutyl phthalate (DBP) impairs spermatogenesis by activating NF-κB/COX-2/RANKL signaling in Sertoli cells.

Toxicology

Xie Z,Liu S,Hua S,Wu L,Zhang Y,Zhu Y,Shi F,Jiang J

Mon May 30 00:00:00 EDT 2022

Preconception exposure to dibutyl phthalate (DBP) impairs spermatogenesis by activating NF-κB/COX-2/RANKL signaling in Sertoli cells.

Toxicology

Xie Z,Liu S,Hua S,Wu L,Zhang Y,Zhu Y,Shi F,Jiang J

Mon May 30 00:00:00 EDT 2022

Saponins from Nigella glandulifera seeds attenuate collagen-induced rheumatoid arthritis in rats via the OPG/RANKL/NF-κB and Ang/Tie-2 pathways.

Journal of ethnopharmacology

Jiang H,Xu F,Zeng L,Li C,Chen Y,Wang L,Li Z,Liu R

Sun Jan 30 00:00:00 EST 2022

Ubiquitin-specific protease 47 is associated with vascular calcification in chronic kidney disease by regulating osteogenic transdifferentiation of vascular smooth muscle cells.

Renal failure

Xiao Q,Tang Y,Xia J,Luo H,Yu M,Chen S,Wang W,Pu L,Wang L,Li G,Li Y

Thu Dec 01 00:00:00 EST 2022

S-Equol enhances osteoblastic bone formation and prevents bone loss through OPG/RANKL via the PI3K/Akt pathway in streptozotocin-induced diabetic rats.

Frontiers in nutrition

Xu Z,Xu J,Li S,Cui H,Zhang G,Ni X,Wang J

Tue Nov 08 00:00:00 EST 2022

Asperuloside Prevents Peri-Implantitis via Suppression of NF-κB and ERK1/2 on Rats.

Pharmaceuticals (Basel, Switzerland)

Wang X,Chen X,Zhang Z,Chen J,Ge Z,Huang S,Wei H,Li D

Sat Aug 20 00:00:00 EDT 2022

Sinomenine Inhibits Orthodontic Tooth Movement and Root Resorption in Rats and Enhances Osteogenic Differentiation of PDLSCs.

Drug design, development and therapy

Li H,Li Y,Zou J,Yang Y,Han R,Zhang J

Tue Sep 13 00:00:00 EDT 2022

Fecal microbiota transplantation ameliorates bone loss in mice with ovariectomy-induced osteoporosis via modulating gut microbiota and metabolic function.

Journal of orthopaedic translation

Zhang YW,Cao MM,Li YJ,Lu PP,Dai GC,Zhang M,Wang H,Rui YF

Tue Nov 01 00:00:00 EDT 2022

Increased expression of osteopontin in subchondral bone promotes bone turnover and remodeling, and accelerates the progression of OA in a mouse model.

Aging

Lin C,Chen Z,Guo D,Zhou L,Lin S,Li C,Li S,Wang X,Lin B,Ding Y

Tue Jan 04 00:00:00 EST 2022

Regulatory roles of miRNAs 16, 133a, and 223 on osteoclastic bone destruction caused by breast cancer metastasis.

International journal of oncology

Kitayama K,Kawamoto T,Kawakami Y,Hara H,Takemori T,Fujiwara S,Yahiro S,Miyamoto T,Mifune Y,Hoshino Y,Kakutani K,Matsumoto T,Matsushita T,Niikura T,Kuroda R,Akisue T

Mon Nov 01 00:00:00 EDT 2021

Metformin attenuates osteoclast-mediated abnormal subchondral bone remodeling and alleviates osteoarthritis via AMPK/NF-κB/ERK signaling pathway.

PloS one

Guo H,Ding D,Wang L,Yan J,Ma L,Jin Q

Mon Jan 10 00:00:00 EST 2022

Anti-osteoporotic effects of alisol C 23-acetate via osteoclastogenesis inhibition.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Jia X,Zhu H,Li G,Lan M,Li X,Huang M,Xu W,Wu S

Sat May 01 00:00:00 EDT 2021

Total Saponins from Nigella glandulifera Seeds Ameliorate Adjuvant-Induced Rheumatoid Arthritis in Rats by Inhibition of an Inflammatory Response and Bone Erosion.

BioMed research international

Zeng L,Li C,Jiang H,Chen Y,Li Z,Xu F,Liu R

Wed May 19 00:00:00 EDT 2021

Milk Polar Lipids Supplementation to Obese Rats During Pregnancy and Lactation Benefited Skeletal Outcomes of Male Offspring.

Molecular nutrition & food research

Han L,Du M,Ren F,Mao X

Sun Aug 01 00:00:00 EDT 2021

Loss of chemerin triggers bone remodeling in vivo and in vitro.

Molecular metabolism

Han L,Zhang Y,Wan S,Wei Q,Shang W,Huang G,Fang P,Min W

Mon Nov 01 00:00:00 EDT 2021

Exosomes derived from osteogenic tumor activate osteoclast differentiation and concurrently inhibit osteogenesis by transferring COL1A1-targeting miRNA-92a-1-5p.

Journal of extracellular vesicles

Yu L,Sui B,Fan W,Lei L,Zhou L,Yang L,Diao Y,Zhang Y,Li Z,Liu J,Hao X

Fri Jan 01 00:00:00 EST 2021

Tomatidine Suppresses the Destructive Behaviors of Fibroblast-Like Synoviocytes and Ameliorates Type II Collagen-Induced Arthritis in Rats.

Frontiers in pharmacology

Yu X,Zhou J,Zhao F,Liu X,Mao Y,Diao L,Wen C,Liu M

Tue Sep 14 00:00:00 EDT 2021

Anti-rheumatoid arthritis effects of iridoid glucosides from Lamiophlomis rotata (Benth.) kudo on adjuvant-induced arthritis in rats by OPG/RANKL/NF-κB signaling pathways.

Journal of ethnopharmacology

Zhao X,Jiang S,Dong Q,Dang J,Liu Z,Han H,Tao Y,Yue H

Wed Feb 10 00:00:00 EST 2021

Knee loading repairs osteoporotic osteoarthritis by relieving abnormal remodeling of subchondral bone via Wnt/β-catenin signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zheng W,Ding B,Li X,Liu D,Yokota H,Zhang P

Sat Feb 01 00:00:00 EST 2020

Glyburide inhibits the bone resorption induced by traumatic occlusion in rats.

Journal of periodontal research

Arita Y,Yoshinaga Y,Kaneko T,Kawahara Y,Nakamura K,Ohgi K,Arita S,Ryu T,Takase M,Sakagami R

Mon Jun 01 00:00:00 EDT 2020

Sialic acid-modified chitosan oligosaccharide-based biphasic calcium phosphate promote synergetic bone formation in rheumatoid arthritis therapy.

Journal of controlled release : official journal of the Controlled Release Society

Xu XL,Shu GF,Wang XJ,Qi J,Jin FY,Shen QY,Ying XY,Ji JS,Du YZ

Fri Jul 10 00:00:00 EDT 2020

MicroRNA‑21 serves an important role during PAOO‑facilitated orthodontic tooth movement.

Molecular medicine reports

Zhang Y,Tian Y,Yang X,Zhao Z,Feng C,Zhang Y

Wed Jul 01 00:00:00 EDT 2020

Gingival crevicular fluid levels of SLIT3 are increased in periodontal disease.

Oral diseases

Zhong W,Peng Y,Yue E,Huang B,Zhang W,Zhao Z,Jiang J,Wang Q,Zhao H

Wed Jan 01 00:00:00 EST 2020

Genistein prevents bone loss in type 2 diabetic rats induced by streptozotocin.

Food & nutrition research

Lu R,Zheng Z,Yin Y,Jiang Z

Tue Apr 19 00:00:00 EDT 2022

The effects of binge alcohol exposure on tooth movement and associated root resorption in rats.

Alcohol (Fayetteville, N.Y.)

Yang F,Wang XX,Li J,Nie FJ,Cui Q,Fu YJ,Zhang J

Sun Nov 01 00:00:00 EDT 2020

Plasma Exosomes Derived From Patients With End-Stage Renal Disease and Renal Transplant Recipients Have Different Effects on Vascular Calcification.

Frontiers in cell and developmental biology

Lin X,Zhu T,Xu F,Zhong JY,Li F,Shan SK,Wu F,Guo B,Zheng MH,Wang Y,Xu QS,Liao XB,Lu HY,Xie XB,Yuan LQ

Tue Feb 16 00:00:00 EST 2021

Fluid Shear Stress Suppresses Osteoclast Differentiation in RAW264.7 Cells through Extracellular Signal-Regulated Kinase 5 (ERK5) Signaling Pathway.

Medical science monitor : international medical journal of experimental and clinical research

Ma C,Geng B,Zhang X,Li R,Yang X,Xia Y

Wed Jan 08 00:00:00 EST 2020

Elevated ATF4 Expression in Odontogenic Keratocysts Epithelia: Potential Involvement in Tissue Hypoxia and Stromal M2 Macrophage Infiltration.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Zhong WQ,Li ZZ,Jiang H,Zou YP,Wang HT,Cai Y,Zhao Y,Zhao JH

Fri Nov 01 00:00:00 EDT 2019

Effects Of Triptolide On Tooth Movement And Root Resorption In Rats.

Drug design, development and therapy

Yang F,Wang XX,Ma D,Cui Q,Zheng H,Liu XC,Zhang J

Wed Jun 03 00:00:00 EDT 2020

LOX-1 is involved in TLR2 induced RANKL regulation in peri-implantitis.

International immunopharmacology

Zhang Q,Liu J,Ma L,Bai N,Xu H

Sun Dec 01 00:00:00 EST 2019

LOX-1 is involved in TLR2 induced RANKL regulation in peri-implantitis.

International immunopharmacology

Zhang Q,Liu J,Ma L,Bai N,Xu H

Sun Dec 01 00:00:00 EST 2019

β-Estradiol antagonizes the inhibitory effects of caffeine in BMMSCs via the ERβ-mediated cAMP-dependent PKA pathway.

Toxicology

Wang C,Zhou Y,Guan X,Yu M,Wang H

Thu Feb 01 00:00:00 EST 2018

β-Estradiol antagonizes the inhibitory effects of caffeine in BMMSCs via the ERβ-mediated cAMP-dependent PKA pathway.

Toxicology

Wang C,Zhou Y,Guan X,Yu M,Wang H

Thu Feb 01 00:00:00 EST 2018

Increased level of cell-derived microparticles in the cyst fluids of odontogenic keratocysts.

International journal of oncology

Man QW,Zhong WQ,Ren JG,Liu JY,Zheng YY,Li RF,Nyimi BF,Chen G,Zhao YF,Liu B

Fri Jun 01 00:00:00 EDT 2018

Increased level of cell-derived microparticles in the cyst fluids of odontogenic keratocysts.

International journal of oncology

Man QW,Zhong WQ,Ren JG,Liu JY,Zheng YY,Li RF,Nyimi BF,Chen G,Zhao YF,Liu B

Fri Jun 01 00:00:00 EDT 2018

Role of endoplasmic reticulum stress in disuse osteoporosis.

Bone

Li J,Yang S,Li X,Liu D,Wang Z,Guo J,Tan N,Gao Z,Zhao X,Zhang J,Gou F,Yokota H,Zhang P

Sat Apr 01 00:00:00 EDT 2017

Role of endoplasmic reticulum stress in disuse osteoporosis.

Bone

Li J,Yang S,Li X,Liu D,Wang Z,Guo J,Tan N,Gao Z,Zhao X,Zhang J,Gou F,Yokota H,Zhang P

Sat Apr 01 00:00:00 EDT 2017

Knee loading inhibits osteoclast lineage in a mouse model of osteoarthritis.

Scientific reports

Li X,Yang J,Liu D,Li J,Niu K,Feng S,Yokota H,Zhang P

Mon Apr 18 00:00:00 EDT 2016

Knee loading inhibits osteoclast lineage in a mouse model of osteoarthritis.

Scientific reports

Li X,Yang J,Liu D,Li J,Niu K,Feng S,Yokota H,Zhang P

Mon Apr 18 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: CD254; H-RANK-L; M-Rank-L; ODF; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; rank-l; RANKL; receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; srankl; TNF-related activation-induced cytokine; TRANCE; tumor necrosis factor (ligand) superfamily member 11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; Tumor necrosis factor ligand superfamily member 11

View more View less

Gene Aliases: CD254; hRANKL2; ODF; OPGL; OPTB2; RANKL; sOdf; TNFSF11; TNLG6B; TRANCE

View more View less

UniProt ID: (Human) O14788

View more View less

Entrez Gene ID: (Human) 8600

View more View less

Function(s)

cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

RANKL Polyclonal Antibody

RANKL Antibody (23408-1-AP) in IHC (P)

Product Details

23408-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!