|Tested species reactivity||Many|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide KYDPTIEDSYRKQVEVDC conjugated to KLH, corresponding to amino acids 31-48 of Human RAP1a.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29451 detects RAP1A from human, mouse, rat, bovine, chicken, canine, guinea pig, Xenopus laevis, hamster, monkey, porcine, rabbit, and sheep samples. It is expected to cross-react with zebrafish and C. elegans based on sequence homology of 100% and 94%, respectively.
PA1-29451 has been successfully used in Western blot applications where a band of 25 kDa is detected. Positive control: rat brain tissue extract.
The PA1-29451 immunogen is a synthetic peptide [KYDPTIEDSYRKQVEVDC] conjugated to KLH, corresponding to amino acids 31-48 of Human RAP1a. This sequence is identical to RAP1b except that RAP1b has an alanine instead of a cysteine at residue 48.
Store at 4°C short term; for long term storage, store at -20°C, avoiding freeze/thaw cycles.
The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.