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          • Primary Antibodies ›
          • RAP1A/RAP1B Antibodies

          Invitrogen

          RAP1A/RAP1B Recombinant Rabbit Monoclonal Antibody (5A1)

          View all (5) RAP1A/RAP1B antibodies

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          Cite RAP1A/RAP1B Recombinant Rabbit Monoclonal Antibody (5A1)

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          • Antibody Testing Data (1)
          RAP1A/RAP1B Antibody in Western Blot (WB)
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          RAP1A/RAP1B Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          RAP1A/RAP1B Antibody (MA573073) in WB

          Western blot analysis of RAP1A/RAP1B in HEK-293T, PC-12, RAW 264.7, Mouse spleen, rat spleen, A-431, HeLa, and Mouse brain at 25 µg/lane. Samples were incubated using RAP1A/RAP1B Recombinant Monoclonal (Product # MA573073) at 1:1,000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          RAP1A/RAP1B Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          MA573073

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Flow Cytometry (Flow)

          1:50-1:100
          -

          Immunoprecipitation (IP)

          1:20-1:50
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Expression System

          HEK293 cells

          Class

          Recombinant Monoclonal

          Type

          Antibody

          Clone

          5A1

          Immunogen

          KLH conjugated synthetic peptide derived from human RAP1A.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.3 mg/mL

          Amount

          30 µg

          Purification

          Protein A

          Storage buffer

          TBS, pH 7.4, with 50% glycerol, 1% BSA

          Contains

          0.02% ProClin 300

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: GTP-binding protein smg p21A; GTP-binding protein smg p21B; GTP-binding protein SMG-P21A; NB4 apoptosis/differentiation related protein; RAP1B; Ras family small GTP binding protein RAP1B; RAS related protein 1b; Ras-like GTPase; RAS-related protein 1a; Ras-related protein Krev-1; RAS-related protein RAP1B; small GTP binding protein; small Ras-like G protein; unnamed protein product

          View more View less

          Gene Aliases: 2810443E11Rik; C21KG; G-22K; K-REV; KREV-1; KREV1; RAL1B; rap 1A; RAP-1A; RAP1; SMGP21; THC11

          View more View less

          UniProt ID: (Human) P62834, (Human) P61224, (Mouse) P62835, (Rat) P62836, (Rat) Q62636, (Mouse) Q99JI6

          View more View less

          Entrez Gene ID: (Human) 5906, (Human) 5908, (Mouse) 109905, (Rat) 295347, (Rat) 171337, (Mouse) 215449

          View more View less

          Function(s)
          GTPase activity obsolete small monomeric GTPase activity guanyl-nucleotide exchange factor activity protein binding GTP binding small GTPase binding macromolecular complex binding GDP binding
          Process(es)
          signal transduction adenylate cyclase-activating G-protein coupled receptor signaling pathway Rap protein signal transduction positive regulation of integrin activation establishment of endothelial barrier cellular response to cAMP modification of postsynaptic structure regulation of cell junction assembly regulation of establishment of cell polarity negative regulation of synaptic vesicle exocytosis small GTPase mediated signal transduction response to carbohydrate positive regulation of neuron projection development negative regulation of collagen biosynthetic process nerve growth factor signaling pathway positive regulation of GTPase activity positive regulation of protein kinase activity positive regulation of glucose import positive regulation of phagocytosis positive regulation of Fc receptor mediated stimulatory signaling pathway positive regulation of ERK1 and ERK2 cascade protein localization to plasma membrane response to antineoplastic agent liver regeneration regulation of neurotransmitter receptor localization to postsynaptic specialization membrane cellular response to forskolin cellular response to nerve growth factor stimulus positive regulation of vasculogenesis cell proliferation negative regulation of calcium ion-dependent exocytosis cellular response to gonadotropin-releasing hormone
          It has to be done as per old AB suggested Products section.

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