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Invitrogen
RAP1GAP Polyclonal Antibody
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Product Details
PA5-29841
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Rap1GAP. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.51 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: mouse brain, rat brain.
Predicted reactivity: Human (99%), Mouse (97%), Rat (98%), Zebrafish (81%), Bovine (97%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Rap1GAP specifically stimulates GTP hydrolytic activity of the monomeric G protein Rap1. Physical interaction between G alpha z, a member of the Gi family of trimeric G proteins, and Rap1GAP blocks the ability of regulators of G protein signaling to stimulate GTP hydrolysis of the alpha subunit, and also attenuates the ability of activated G alpha z to inhibit adenylyl cyclase. Rap1GAP is expressed in the brain, kidney and pancreas and may act as a signal integrator to coordinate and/or integrate Gz signaling and Rap1 signaling in cells. A novel isoform of Rapl GTPase-activating protein, designated Rap1GAPII, binds specifically to G alpha z. Stimulation of the Gi-coupled M2 Muscarinic receptor translocates Rap1GAPII from the cytosol to the membrane and decreases the amount of GTP-bound Rap1, resulting in the activation of ERK/MAPK.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARPP-90; Rap1 GTPase-activating protein 1; Rap1, GTPase-activating protein 1; Rap1GAP
Gene Aliases: 1300019I11Rik; 2310004O14Rik; AI427470; ARPP-90; KIAA0474; RAP1GA1; RAP1GAP; RAP1GAP1; RAP1GAPII; RAPGAP
UniProt ID: (Human) P47736, (Mouse) A2ALS5
Entrez Gene ID: (Human) 5909, (Mouse) 110351, (Rat) 313644
signal transduction
regulation of GTPase activity
positive regulation of GTPase activity
negative regulation of neuron differentiation
regulation of small GTPase mediated signal transduction
negative regulation of microvillus assembly
negative regulation of GTP binding
negative regulation of thyroid gland epithelial cell proliferation
cellular response to glial cell derived neurotrophic factor
negative regulation of cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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