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Bethyl Laboratories
RAP80 Polyclonal Antibody
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FIGURE: 1 / 1
RAP80 Antibody (A300-763A) in WB
Detection of human RAP80 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RAP80 antibody (Product # A300-763A (Product # A300-763A-2) used for WB at 0.1 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. RAP80 was also immunoprecipitated using a previous lot (Product # A300-763A-1) of this antibody and by rabbit anti-RAP80 antibody A300-764A, which recognizes ... View More
Product Details
A300-763A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 600 and 650 of Human Receptor Associated Protein 80.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BRCA1-A complex subunit RAP80; Receptor-associated protein 80; Retinoid X receptor-interacting protein 110; RIP110; Ubiquitin interaction motif-containing protein 1; unnamed protein product
Gene Aliases: RAP80; RXRIP110; UIMC1; X2HRIP110
UniProt ID: (Human) Q96RL1
Entrez Gene ID: (Human) 51720
DNA repair
regulation of DNA repair
double-strand break repair
chromatin organization
cellular response to DNA damage stimulus
mitotic G2 DNA damage checkpoint
response to ionizing radiation
mitotic G2/M transition checkpoint
positive regulation of DNA repair
negative regulation of transcription, DNA-templated
DNA repair-dependent chromatin remodeling
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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