Immunofluorescent analysis of RARB showing staining in the nucleus of Hela cells using a RARB polyclonal antibody (Product # PA5-35206) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/ml, 1 h at 37°C). Nuclei were stained with DAPI (blue) (10 ug/ml, 10 min).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 168-195 amino acids from the central region of human RARB|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chicken and mouse based on sequence homology.
This gene encodes retinoic acid receptor beta, a member ofthe thyroid-steroid hormone receptor superfamily of nucleartranscriptional regulators. This receptor localizes to thecytoplasm and to subnuclear compartments. It binds retinoic acid,the biologically active form of vitamin A which mediates cellularsignalling in embryonic morphogenesis, cell growth anddifferentiation. It is thought that this protein limits growth ofmany cell types by regulating gene expression. The gene was firstidentified in a hepatocellular carcinoma where it flanks ahepatitis B virus integration site. The gene expresses at least twotranscript variants; one additional transcript has been described,but its full length nature has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.