Invitrogen

RASGRP1 Polyclonal Antibody

Product Image Gallery

RASGRP1 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human brain tissue using a RASGRP1 polyclonal antibody (Product # PA5-25750), followed by HRP-conjugated secondary antibody and DAB staining.

RASGRP1 Antibody in Western Blot (WB) — Western blot analysis using a RASGRP1 polyclonal antibody (Product # PA5-25750) in HepG2 cell lysates (35 µg per lane).

RASGRP1 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of HepG2 cells using a RASGRP1 polyclonal antibody (Product # PA5-25750) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Product Details

PA5-25750

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:10-1:50	-
Flow Cytometry (Flow)	1:10-1:50	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / Ig
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 495-521 amino acids from the central region of human RASGRP1 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.8 mg/mL
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2543250

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

Functions as a calcium- and diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Regulates T-cell/B-cell development, homeostasis and differentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras. Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways. Functions in mast cell degranulation and cytokine secretion, regulating FcERI-evoked allergic responses. May also function in differentiation of other cell types.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of RASGRP1 in humans impairs T-cell expansion leading to Epstein-Barr virus susceptibility.

EMBO molecular medicine

Winter S,Martin E,Boutboul D,Lenoir C,Boudjemaa S,Petit A,Picard C,Fischer A,Leverger G,Latour S

PA5-25750 was used in Western Blotting to demonstrate how RASGRP1 plays a key role in the expansion of activated T lymphocytes and how its deficiency leads to susceptibility to Epstein-Barr virus.

Thu Feb 01 00:00:00 EST 2018

Loss of RASGRP1 in humans impairs T-cell expansion leading to Epstein-Barr virus susceptibility.

EMBO molecular medicine

Winter S,Martin E,Boutboul D,Lenoir C,Boudjemaa S,Petit A,Picard C,Fischer A,Leverger G,Latour S

PA5-25750 was used in Western Blotting to demonstrate how RASGRP1 plays a key role in the expansion of activated T lymphocytes and how its deficiency leads to susceptibility to Epstein-Barr virus.

Thu Feb 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: Calcium and DAG-regulated guanine nucleotide exchange factor II; calcium- and diacylglycerol-regulated guanine nucleotide exchange factor II; CalDAG-GEFII; guanine nucleotide exchange factor, calcium- and DAG-regulated, Rap1A; ras activator RasGRP; RAS guanyl nucleotide-releasing protein 1; RAS guanyl releasing protein 1 (calcium and DAG-regulated); Ras guanyl-releasing protein; RAS guanyl-releasing protein 1

Gene Aliases: CALDAG-GEFI; CALDAG-GEFII; hRasGRP1; RASGRP; RASGRP1; V

UniProt ID: (Human) O95267

Entrez Gene ID: (Human) 10125

Molecular Function: guanyl-nucleotide exchange factor