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Product Details
PA5-87944
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 598-797 of human RASGRP1 (NP_0057302)
Conjugate
Form
Liquid
Concentration
2.48 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804526
Product Specific Information
Immunogen sequence: PVAPTENNTS VGPVSNLCSL GAKDLLHAPE EGPFTFPNGE AVEHGEESKD RTIMLMGVSS QKISLRLKRA VAHKATQTES QPWIGSEGPS GPFVLSSPRK TAQDTLYVLP SPTSPCPSPV LVRKRAFVKW ENKDSLIKSK EELRHLRLPT YQELEQEINT LKADNDALKI QLKYAQKKIE SLQLEKSNHV LAQMEQGDCS; Positive Samples: Jurkat, MCF7, A-549, U-251MG, SW480; Cellular Location: Cell membrane, Cytoplasm, Endoplasmic reticulum membrane, Golgi apparatus membrane, Peripheral membrane protein, cytosol
Target Information
Functions as a calcium- and diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. Activates the Erk/MAP kinase cascade. Regulates T-cell/B-cell development, homeostasis and differentiation by coupling T-lymphocyte/B-lymphocyte antigen receptors to Ras. Regulates NK cell cytotoxicity and ITAM-dependent cytokine production by activation of Ras-mediated ERK and JNK pathways. Functions in mast cell degranulation and cytokine secretion, regulating FcERI-evoked allergic responses. May also function in differentiation of other cell types.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Calcium and DAG-regulated guanine nucleotide exchange factor II; calcium- and diacylglycerol-regulated guanine nucleotide exchange factor II; CalDAG-GEF1; CalDAG-GEFII; guanine nucleotide exchange factor, calcium- and DAG-regulated, Rap1A; ras activator RasGRP; RAS guanyl nucleotide-releasing protein 1; RAS guanyl releasing protein 1 (calcium and DAG-regulated); Ras guanyl-releasing protein; RAS guanyl-releasing protein 1
Gene Aliases: CALDAG-GEFI; CALDAG-GEFII; hRasGRP1; RASGRP; RASGRP1; V
UniProt ID: (Human) O95267, (Rat) Q9R1K8, (Mouse) Q9Z1S3
Entrez Gene ID: (Human) 10125, (Rat) 29434, (Mouse) 19419
phosphatidylserine binding
guanyl-nucleotide exchange factor activity
Ras guanyl-nucleotide exchange factor activity
calcium ion binding
zinc ion binding
lipid binding
diacylglycerol binding
phosphatidylcholine binding
protein homodimerization activity
Rap guanyl-nucleotide exchange factor activity
metal ion binding
guanyl-nucleotide exchange factor
MAPK cascade
cytokine production
positive regulation of protein phosphorylation
inflammatory response to antigenic stimulus
signal transduction
Ras protein signal transduction
regulation of phosphatidylinositol 3-kinase signaling
cell differentiation
secretory granule localization
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of tumor necrosis factor production
positive regulation of natural killer cell differentiation
positive regulation of T cell differentiation in thymus
mast cell degranulation
positive regulation of MAP kinase activity
positive regulation of GTPase activity
positive regulation of natural killer cell mediated cytotoxicity
positive regulation of JNK cascade
positive regulation of Ras protein signal transduction
vesicle transport along microtubule
protein oligomerization
regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
activation of GTPase activity
regulation of phospholipase C activity
regulation of p38MAPK cascade
positive regulation of interferon-gamma secretion
small GTPase mediated signal transduction
intracellular signal transduction
regulation of GTPase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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