Invitrogen

RASSF2 Polyclonal Antibody

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RASSF2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of RASSF2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with RASSF2 polyclonal antibody (Product # PA5-103099) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

RASSF2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded RASSF2 in human lung tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with RASSF2 polyclonal antibody (Product # PA5-103099) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

RASSF2 Antibody in Western Blot (WB) — Western blot analysis of RASSF2 in HepG2 cell lysate. Samples were incubated with RASSF2 polyclonal antibody (Product # PA5-103099).

Product Details

PA5-103099

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human RASSF2(Accession P50749), corresponding to amino acid residues P122-V149. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2852469

Target Information

Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: centromere protein 34; Ras association (RalGDS/AF-6) domain family 2; Ras association (RalGDS/AF-6) domain family member 2; Ras association domain-containing protein 2; RP4-599I11.1

Gene Aliases: 3830431H01Rik; 9030412M04Rik; AI852669; AW495050; CENP-34; KIAA0168; RASFADIN; RASSF2

UniProt ID: (Human) P50749, (Mouse) Q8BMS9, (Rat) Q3B7D5

Entrez Gene ID: (Human) 9770, (Mouse) 215653, (Rat) 311437

Molecular Function: scaffold/adaptor protein