Active Motif

RBBP5 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

RBBP5 Antibody in Western Blot (WB) — RBBP5 antibody (pAb) tested by Western blot. MCF-7 whole-cell extract (20 µg) probed with RBBP5 antibody at a dilution of 1:1,000.

RBBP5 Antibody in ChIP-sequencing (ChIP-Seq) — RBBP5 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 ug of human PBMC chromatin and 7 µl of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 21 million sequence tags were mapped to identify RBBP5 binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.

Product Details

61405

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	-
ChIP assay (ChIP)	10 µL	-
ChIP-sequencing (ChIP-Seq)	10 µL	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	This antibody was raised against a peptide within the C-terminal region of human RBBP5. View immunogen
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.5, with 30% glycerol
Contains	0.035% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2793622

Target Information

This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The metabolic sensor PASK is a histone 3 kinase that also regulates H3K4 methylation by associating with H3K4 MLL2 methyltransferase complex.

Nucleic acids research

Karakkat JV,Kaimala S,Sreedharan SP,Jayaprakash P,Adeghate EA,Ansari SA,Guccione E,Mensah-Brown EPK,Starling Emerald B

Research topic: Histone Methylation

Mon Nov 04 00:00:00 EST 2019

Bioinformatics

Protein Aliases: RBBP 5; RBBP-5; RBQ 3; retinoblastoma binding protein 5; Retinoblastoma-binding protein 5; Retinoblastoma-binding protein RBQ-3; SWD1, Set1c WD40 repeat protein, homolog

Gene Aliases: RBBP5; RBQ3; SWD1

UniProt ID: (Human) Q15291

Entrez Gene ID: (Human) 5929

Function(s)

transcription, DNA-templated regulation of transcription, DNA-templated cellular response to DNA damage stimulus response to estrogen histone H3-K4 methylation beta-catenin-TCF complex assembly

Process(es)

protein binding histone-lysine N-methyltransferase activity methylated histone binding histone methyltransferase activity (H3-K4 specific) transcription regulatory region DNA binding