Proteintech

RBM15 Polyclonal Antibody

RBM15 Antibody in Immunocytochemistry (ICC/IF)

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RBM15 Antibody (10587-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using RBM15 antibody 10587-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

RBM15 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RBM15 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10587-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 4 publications 4 publications

RNA Immunoprecipitation (RIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RBM15 Fusion Protein Ag0938 (677-977 aa encoded by BC006397)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.32 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: SSSRDRYNS DNDRSSRLLL ERPSPIRDRR GSLEKSQGDK RDRKNSASAE RDRKHRTTAP TEGKSPLKKE DRSDGSAPST STASSKLKSP SQKQDGGTAP VASASPKLCL AWQGMLLLKN SNFPSNMHLL QGDLQVASSL LVEGSTGGKV AQLKITQRLR LDQPKLDEVT RRIKVAGPNG YAILLAVPGS SDSRSSSSSA ASDTATSTQR PLRNLVSYLK QKQAAGVISL PVGGNKDKEN TGVLHAFPPC EFSQQFLDSP AKALAKSEED YLVMIIVRGF GFQIGVRYEN KKRENLALTL L (677-977 aa encoded by BC006397)

Target Information

May function as an mRNA export factor, stimulating export and expression of RTE-containing mRNAs which are present in many retrotransposons that require to be exported prior to splicing. High affinity binding of pre-mRNA to RBM15 may allow targeting of the mRNP to the export helicase DBP5 in a manner that is independent of splicing-mediated NXF1 deposition, resulting in export prior to splicing. May be implicated in HOX gene regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

FTO alleviates cerebral ischemia/reperfusion-induced neuroinflammation by decreasing cGAS mRNA stability in an m6A-dependent manner.

Cellular signalling

Yu Z,Zheng L,Geng Y,Zhang Y,Wang Y,You G,Cai M,Li M,Cheng X,Zan J

Fri Sep 01 00:00:00 UTC 2023

RNA methylation pattern and immune microenvironment characteristics mediated by m6A regulator in ischemic stroke.

Frontiers in genetics

Jia K,Xia W,Su Q,Yang S,Zhang Y,Ni X,Su Z,Meng D

Fri May 05 00:00:00 UTC 2023

Identification of osteosarcoma m6A-related prognostic biomarkers using artificial intelligence: RBM15.

Scientific reports

Jiang J,Qu H,Zhan X,Liu D,Liang T,Chen L,Huang S,Sun X,Chen J,Chen T,Li H,Yao Y,Liu C

Fri Mar 31 00:00:00 UTC 2023

RBM15 suppresses hepatic insulin sensitivity of offspring of gestational diabetes mellitus mice via m6A-mediated regulation of CLDN4.

Molecular medicine (Cambridge, Mass.)

Fang J,Wu X,He J,Zhang H,Chen X,Zhang H,Novakovic B,Qi H,Yu X

Mon Feb 20 00:00:00 UTC 2023

RBM15 suppresses hepatic insulin sensitivity of offspring of gestational diabetes mellitus mice via m6A-mediated regulation of CLDN4.

Molecular medicine (Cambridge, Mass.)

Fang J,Wu X,He J,Zhang H,Chen X,Zhang H,Novakovic B,Qi H,Yu X

Mon Feb 20 00:00:00 UTC 2023

RBM15 suppresses hepatic insulin sensitivity of offspring of gestational diabetes mellitus mice via m6A-mediated regulation of CLDN4.

Molecular medicine (Cambridge, Mass.)

Fang J,Wu X,He J,Zhang H,Chen X,Zhang H,Novakovic B,Qi H,Yu X

Mon Feb 20 00:00:00 UTC 2023

RBM15 suppresses hepatic insulin sensitivity of offspring of gestational diabetes mellitus mice via m6A-mediated regulation of CLDN4.

Molecular medicine (Cambridge, Mass.)

Fang J,Wu X,He J,Zhang H,Chen X,Zhang H,Novakovic B,Qi H,Yu X

Mon Feb 20 00:00:00 UTC 2023

The differences in biological behavior and gene expression characteristics between pure and mixed early gastric signet ring cell carcinomas.

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver

Wang X,Li X,Yang P,Qin X,Yu J,Cheng Y,Zhang G,Tang L

Thu Jun 01 00:00:00 UTC 2023

m6A Regulatory Gene-Mediated Methylation Modification in Glioma Survival Prediction.

Frontiers in genetics

Zhang G,Zheng P,Lv Y,Shi Z,Shi F

Mon Jan 09 00:00:00 UTC 2023

Cross talk between RNA modification writers and tumor development as a basis for guiding personalized therapy of gastric cancer.

Human genomics

Zhang S,Kuang G,Huang Y,Huang X,Wang W,Wang G

Fri Apr 22 00:00:00 UTC 2022

Analysis and identification of m⁶A RNA methylation regulators in metastatic osteosarcoma.

Molecular therapy. Nucleic acids

Huang H,Cui X,Qin X,Li K,Yan G,Lu D,Zheng M,Hu Z,Lei D,Lan N,Zheng L,Yuan Z,Zhu B,Zhao J

Tue Mar 08 00:00:00 UTC 2022

Analysis and identification of m⁶A RNA methylation regulators in metastatic osteosarcoma.

Molecular therapy. Nucleic acids

Huang H,Cui X,Qin X,Li K,Yan G,Lu D,Zheng M,Hu Z,Lei D,Lan N,Zheng L,Yuan Z,Zhu B,Zhao J

Tue Mar 08 00:00:00 UTC 2022

Systematic analysis of the expression profile and prognostic significance of m6A regulators and PD-L1 in hepatocellular carcinoma.

Discover. Oncology

Kong F,Wang K,Wang L

Fri Nov 25 00:00:00 UTC 2022

Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling.

Journal of clinical laboratory analysis

Li Z,Xu Q,Huangfu N,Chen X,Zhu J

Sat Jan 01 00:00:00 UTC 2022

YTHDC1 regulates distinct post-integration steps of HIV-1 replication and is important for viral infectivity.

Retrovirology

N'Da Konan S,Ségéral E,Bejjani F,Bendoumou M,Ait Said M,Gallois-Montbrun S,Emiliani S

Mon Jan 31 00:00:00 UTC 2022

Integrated analysis of m⁶A mRNA methylation in rats with monocrotaline-induced pulmonary arterial hypertension.

Aging

Zeng Y,Huang T,Zuo W,Wang D,Xie Y,Wang X,Xiao Z,Chen Z,Liu Q,Liu N,Xiao Y

Mon Jul 26 00:00:00 UTC 2021

Methylation and Expression of the Exercise-Related TLR1 Gene Is Associated With Low Grade Glioma Prognosis and Outcome.

Frontiers in molecular biosciences

Lu L,Hu Y,Wang C,Jiang F,Wu C

Tue Dec 07 00:00:00 UTC 2021

RBM15 facilitates laryngeal squamous cell carcinoma progression by regulating TMBIM6 stability through IGF2BP3 dependent.

Journal of experimental & clinical cancer research : CR

Wang X,Tian L,Li Y,Wang J,Yan B,Yang L,Li Q,Zhao R,Liu M,Wang P,Sun Y

Fri Feb 26 00:00:00 UTC 2021

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Nature

Xu W,Li J,He C,Wen J,Ma H,Rong B,Diao J,Wang L,Wang J,Wu F,Tan L,Shi YG,Shi Y,Shen H

Mon Mar 01 00:00:00 UTC 2021

N⁶-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles.

Developmental cell

Wang X,Liu C,Zhang S,Yan H,Zhang L,Jiang A,Liu Y,Feng Y,Li D,Guo Y,Hu X,Lin Y,Bu P,Li D

Mon Mar 08 00:00:00 UTC 2021

N⁶-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles.

Developmental cell

Wang X,Liu C,Zhang S,Yan H,Zhang L,Jiang A,Liu Y,Feng Y,Li D,Guo Y,Hu X,Lin Y,Bu P,Li D

Mon Mar 08 00:00:00 UTC 2021

N⁶-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles.

Developmental cell

Wang X,Liu C,Zhang S,Yan H,Zhang L,Jiang A,Liu Y,Feng Y,Li D,Guo Y,Hu X,Lin Y,Bu P,Li D

Mon Mar 08 00:00:00 UTC 2021

N⁶-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles.

Developmental cell

Wang X,Liu C,Zhang S,Yan H,Zhang L,Jiang A,Liu Y,Feng Y,Li D,Guo Y,Hu X,Lin Y,Bu P,Li D

Mon Mar 08 00:00:00 UTC 2021

mTORC1 stimulates cell growth through SAM synthesis and m⁶A mRNA-dependent control of protein synthesis.

Molecular cell

Villa E,Sahu U,O'Hara BP,Ali ES,Helmin KA,Asara JM,Gao P,Singer BD,Ben-Sahra I

Thu May 20 00:00:00 UTC 2021

Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.

Medical science monitor : international medical journal of experimental and clinical research

Zhang Y,Liu X,Liu L,Li J,Hu Q,Sun R

Sat Feb 22 00:00:00 UTC 2020

The role of the Xist 5' m6A region and RBM15 in X chromosome inactivation.

Wellcome open research

Coker H,Wei G,Moindrot B,Mohammed S,Nesterova T,Brockdorff N

Mon Sep 28 00:00:00 UTC 2020

RBM15 Modulates the Function of Chromatin Remodeling Factor BAF155 Through RNA Methylation in Developing Cortex.

Molecular neurobiology

Xie Y,Castro-Hernández R,Sokpor G,Pham L,Narayanan R,Rosenbusch J,Staiger JF,Tuoc T

Fri Nov 01 00:00:00 UTC 2019

Splicing factor SF3B1K700E mutant dysregulates erythroid differentiation via aberrant alternative splicing of transcription factor TAL1.

PloS one

Jin S,Su H,Tran NT,Song J,Lu SS,Li Y,Huang S,Abdel-Wahab O,Liu Y,Zhao X

Fri Sep 08 00:00:00 UTC 2017

Splicing factor SF3B1K700E mutant dysregulates erythroid differentiation via aberrant alternative splicing of transcription factor TAL1.

PloS one

Jin S,Su H,Tran NT,Song J,Lu SS,Li Y,Huang S,Abdel-Wahab O,Liu Y,Zhao X

Fri Sep 08 00:00:00 UTC 2017

Multiple regions of Kaposi's sarcoma-associated herpesvirus ORF59 RNA are required for its expression mediated by viral ORF57 and cellular RBM15.

Viruses

Massimelli MJ,Majerciak V,Kang JG,Liewehr DJ,Steinberg SM,Zheng ZM

Tue Feb 03 00:00:00 UTC 2015

Multiple regions of Kaposi's sarcoma-associated herpesvirus ORF59 RNA are required for its expression mediated by viral ORF57 and cellular RBM15.

Viruses

Massimelli MJ,Majerciak V,Kang JG,Liewehr DJ,Steinberg SM,Zheng ZM

Tue Feb 03 00:00:00 UTC 2015

A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing.

Cell reports

Moindrot B,Cerase A,Coker H,Masui O,Grijzenhout A,Pintacuda G,Schermelleh L,Nesterova TB,Brockdorff N

Tue Jul 28 00:00:00 UTC 2015

A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing.

Cell reports

Moindrot B,Cerase A,Coker H,Masui O,Grijzenhout A,Pintacuda G,Schermelleh L,Nesterova TB,Brockdorff N

Tue Jul 28 00:00:00 UTC 2015

Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.

eLife

Zhang L,Tran NT,Su H,Wang R,Lu Y,Tang H,Aoyagi S,Guo A,Khodadadi-Jamayran A,Zhou D,Qian K,Hricik T,Côté J,Han X,Zhou W,Laha S,Abdel-Wahab O,Levine RL,Raffel G,Liu Y,Chen D,Li H,Townes T,Wang H,Deng H,Zheng YG,Leslie C,Luo M,Zhao X

Tue Nov 17 00:00:00 UTC 2015

Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.

eLife

Tue Nov 17 00:00:00 UTC 2015

Attenuation of the suppressive activity of cellular splicing factor SRSF3 by Kaposi sarcoma-associated herpesvirus ORF57 protein is required for RNA splicing.

RNA (New York, N.Y.)

Majerciak V,Lu M,Li X,Zheng ZM

Sat Nov 01 00:00:00 UTC 2014

Attenuation of the suppressive activity of cellular splicing factor SRSF3 by Kaposi sarcoma-associated herpesvirus ORF57 protein is required for RNA splicing.

RNA (New York, N.Y.)

Majerciak V,Lu M,Li X,Zheng ZM

Sat Nov 01 00:00:00 UTC 2014

Rbm15-Mkl1 interacts with the Setd1b histone H3-Lys4 methyltransferase via a SPOC domain that is required for cytokine-independent proliferation.

PloS one

Lee JH,Skalnik DG

Tue Jan 29 00:00:00 UTC 2013

Rbm15-Mkl1 interacts with the Setd1b histone H3-Lys4 methyltransferase via a SPOC domain that is required for cytokine-independent proliferation.

PloS one

Lee JH,Skalnik DG

Tue Jan 29 00:00:00 UTC 2013

Rbm15-Mkl1 interacts with the Setd1b histone H3-Lys4 methyltransferase via a SPOC domain that is required for cytokine-independent proliferation.

PloS one

Lee JH,Skalnik DG

Tue Jan 29 00:00:00 UTC 2013

Rbm15-Mkl1 interacts with the Setd1b histone H3-Lys4 methyltransferase via a SPOC domain that is required for cytokine-independent proliferation.

PloS one

Lee JH,Skalnik DG

Tue Jan 29 00:00:00 UTC 2013

Kaposi's sarcoma-associated herpesvirus ORF57 interacts with cellular RNA export cofactors RBM15 and OTT3 to promote expression of viral ORF59.

Journal of virology

Majerciak V,Uranishi H,Kruhlak M,Pilkington GR,Massimelli MJ,Bear J,Pavlakis GN,Felber BK,Zheng ZM

Tue Feb 01 00:00:00 UTC 2011

Kaposi's sarcoma-associated herpesvirus ORF57 interacts with cellular RNA export cofactors RBM15 and OTT3 to promote expression of viral ORF59.

Journal of virology

Majerciak V,Uranishi H,Kruhlak M,Pilkington GR,Massimelli MJ,Bear J,Pavlakis GN,Felber BK,Zheng ZM

Tue Feb 01 00:00:00 UTC 2011

Requirement of UAP56, URH49, RBM15, and OTT3 in the expression of Kaposi sarcoma-associated herpesvirus ORF57.

Virology

Majerciak V,Deng M,Zheng ZM

Thu Nov 25 00:00:00 UTC 2010

Requirement of UAP56, URH49, RBM15, and OTT3 in the expression of Kaposi sarcoma-associated herpesvirus ORF57.

Virology

Majerciak V,Deng M,Zheng ZM

Thu Nov 25 00:00:00 UTC 2010

Nuclear export factor RBM15 facilitates the access of DBP5 to mRNA.

Nucleic acids research