Invitrogen

RBMX Polyclonal Antibody

Product Image Gallery

RBMX Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of RBMX in K-562 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with RBMX polyclonal antibody (Product # PA5-99433) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

RBMX Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded RBMX in human Breast tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with RBMX polyclonal antibody (Product # PA5-99433) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

RBMX Antibody in Western Blot (WB) — Western blot analysis of RBMX in various samples (Lane 1: 293 treated with blocking peptide, Lane 2: 293, Lane 3: HeLa). Samples were incubated with RBMX polyclonal antibody (Product # PA5-99433).

Product Details

PA5-99433

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human hnRNP G, corresponding to a region within N-terminal amino acids. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2818366

Target Information

hnRNP G is a glycoprotein, which was originally identified as an autoantigen from German shepherd dogs with lupus-like syndrome. The gene encoding hnRNP G is located on chromosome Xq26 and is ubiquitously expressed. It contains one RNP-consensus RNA binding domain (RBD) and is related to RBMY, which is involved in spermatogenesis, and hnRNP G-T, which is a testis specific protein. All three proteins interact with Tra2b and therefore, are involved in pre-mRNA splicing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: Glycoprotein p43; Heterogeneous nuclear ribonucleoprotein G; hnRNP G; RNA binding motif protein, X chromosome; RNA-binding motif protein, X chromosome; RNA-binding motif protein, X chromosome retrogene; RNA-binding motif protein, X chromosome retrogene-like; RNA-binding motif protein, X chromosome, N-terminally processed

Gene Aliases: hnRNP G; hnRNP-G; HNRNPG; HNRPG; MRXS11; RBMX; RBMXP1; RBMXRT; RNMX

UniProt ID: (Human) P38159, (Mouse) Q9WV02, (Rat) Q4V898

Entrez Gene ID: (Human) 27316, (Mouse) 19655, (Rat) 302855

Molecular Function: RNA splicing factor