Immunofluorescent analysis of RBMY1F showing staining in the nucleus of NT2D1 cells. NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a RBMY1F polyclonal antibody (Product # PA5-32067) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 155 and 429 of Human RBMY1F|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-32067 targets RBMY1F in WB applications and shows reactivity with Human samples.
The PA5-32067 immunogen is recombinant protein fragment corresponding to a region within amino acids 155 and 429 of Human RBMY1F.
This gene encodes a protein containing an RNA-binding motif in the N-terminus and four SRGY (serine, arginine, glycine, tyrosine) boxes in the C-terminus. Multiple copies of this gene are found in the AZFb azoospermia factor region of chromosome Y and the encoded protein is thought to be involved in spermatogenesis. Most copies of this locus are pseudogenes, although six highly similar copies have full-length ORFs and are considered functional. Four functional copies of this gene are found within inverted repeat IR2; two functional copies of this gene are found in palindrome P3, along with two copies of PTPN13-like, Y-linked.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.