Invitrogen

RBPMS Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

View all (30) RBPMS antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

RBPMS Antibody in Immunofluorescence (IF) — Immunofluorescent staining of RBPMS in human cell line A549 shows positivity in cytoplasm and nucleus but excluded from the nucleoli. Samples were probed using a RBPMS Polyclonal Antibody (Product # PA5-63383).

RBPMS Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of RBPMS in human endometrium and skin tissues using RBPMS Polyclonal Antibody (Product # PA5-63383). Corresponding RBPMS RNA-seq data are presented for the same tissues.

RBPMS Antibody in Western Blot (WB) — Western blot was performed using Anti-RBPMS Polyclonal Antibody (Product # PA5-63383) and a 24 kDa doublet corresponding to RBPMS were observed across cell line tissues tested. Whole cell extracts (30 µg lysate) of BeWo (Lane 1), NTERA-2.cl.D1 (Lane 2) and HeLa (Lane 3) were electrophoresed using Novex® NuPAGE® 12% Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by XCell SureLock™ Mini-Cell and XCell II™ Blot Module (Product # EI0002). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

RBPMS Antibody in Relative expression — Relative expression in western blot: Antibody specificity was demonstrated by detection of known differential basal expression of the target across tissue/cell models. Expression of RBPMS was observed specifically in RT-4 cells and in U-251MG cells using anti-RBPMS Polyclonal Antibody (Product # PA5-63383) in western blot. The relative expression levels of RBPMS within each cell line is shown using RNA-Seq. {RE}

RBPMS Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. NTERA-2.cl.D1 cells were transfected with RBPMS siRNA and reduction in signal was observed in Western Blot using RBPMS Polyclonal Antibody (Product # PA5-63383). {KD}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

0.25-2 µg/mL

Immunofluorescence (IF)

0.25-2 µg/mL

Immunohistochemistry (IHC)

1:50-1:200

Western Blot (WB)

0.04-0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human RBPMS

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2646415

Product Specific Information

Immunogen sequence: PSTPLPNTVP QFIAREPYEL TVPALYPSSP EV

Highest antigen sequence identity to the following orthologs: Mouse - 100%, Rat - 100%.

Target Information

This gene encodes a member of the RRM family of RNA-binding proteins. The RRM domain is between 80-100 amino acids in length and family members contain one to four copies of the domain. The RRM domain consists of two short stretches of conserved sequence called RNP1 and RNP2, as well as a few highly conserved hydrophobic residues. The protein encoded by this gene has a single, putative RRM domain in its N-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.