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Invitrogen
RC3H1 Polyclonal Antibody
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FIGURE: 1 / 2
RC3H1 Antibody (PA5-85598) in WB
RC3H1 Polyclonal Antibody detects RC3H1 protein by western blot analysis. Non-transfected (-) and RC3H1-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with RC3H1 Polyclonal Antibody (Product # PA5-85598) diluted by 1:1,000.
Product Details
PA5-85598
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human RC3H1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (91%).
Positive Control: 293T, A431, HeLa, HepG2, 3xFlag-human RC3H1 transfected 293T.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The ring finger protein RC3H1, also known as Roquin, is a highly conserved member of the RING type ubiquitin ligase protein family whose M199R mutation leads to the excessive production of follicular helper T cells and germinal centers in the sanroque strain of mice, a strain with excessive IL-21 production and high titers of autoantibodies. The complete loss of RC3H1 induces early death and immune deregulation but not autoimmunity in RC3H1-null mice, suggesting that the mutant RC3H1 is more disruptive to the immune system than its complete loss.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: probable E3 ubiquitin-protein ligase Roquin; RING finger and C3H zinc finger protein 1; RING finger and CCCH-type zinc finger domain-containing protein 1; RING finger protein 198; Roquin; Roquin-1
Gene Aliases: KIAA2025; RC3H1; RNF198; ROQUIN
UniProt ID: (Human) Q5TC82
Entrez Gene ID: (Human) 149041
nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
nuclear-transcribed mRNA catabolic process
B cell homeostasis
regulation of germinal center formation
negative regulation of germinal center formation
posttranscriptional regulation of gene expression
protein ubiquitination
negative regulation of B cell proliferation
cytoplasmic mRNA processing body assembly
T cell proliferation
T cell homeostasis
regulation of mRNA stability
negative regulation of T-helper cell differentiation
negative regulation of activated T cell proliferation
lymph node development
spleen development
regulation of T cell receptor signaling pathway
positive regulation of mRNA catabolic process
3'-UTR-mediated mRNA destabilization
T follicular helper cell differentiation
cellular response to interleukin-1
positive regulation of NIK/NF-kappaB signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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