Keep as concentrated solution.
Positive Control: 293T, A431, HeLa, HepG2, 3xFlag-human RC3H1 transfected 293T.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
The ring finger protein RC3H1, also known as Roquin, is a highly conserved member of the RING type ubiquitin ligase protein family whose M199R mutation leads to the excessive production of follicular helper T cells and germinal centers in the sanroque strain of mice, a strain with excessive IL-21 production and high titers of autoantibodies. The complete loss of RC3H1 induces early death and immune deregulation but not autoimmunity in RC3H1-null mice, suggesting that the mutant RC3H1 is more disruptive to the immune system than its complete loss.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: probable E3 ubiquitin-protein ligase Roquin; RING finger and C3H zinc finger protein 1; ring finger and CCCH-type domains 1 Antibody; RING finger and CCCH-type zinc finger domain-containing protein 1; RING finger protein 198; RNF198 Antibody; Roquin; ROQUIN Antibody; Roquin-1; RP5-1198E17.5 Antibody
Gene Aliases: KIAA2025; RC3H1; RNF198; ROQUIN
UniProt ID: (Human) Q5TC82
Entrez Gene ID: (Human) 149041