|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human RCAS-1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.
RCAS1 (Receptor binding cancer antigen expressed on SiSo cells, Estrogen receptor-binding fragment-associated gene 9 protein, BAG9) is a type III transmembrane protein that is predominantly located in the Golgi. It induces apoptosis of natural killer (NK)/T cells and plays a role in immune invasion, possibly in relation to Epstein-Bar virus infection. RCAS1 is abundantly expressed in prostate cancer cells when compared to normal epithelial cells, and immunodetection of RCAS1 expression can be a negative prognostic indicator for patients with prostate cancer
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The role of RCAS1 as a biomarker in diagnosing CRC and monitoring tumor recurrence and metastasis.
PA5-32571 was used in immunohistochemistry and western blot to evaluate the diagnostic and prognostic value of RCAS1 as a biomarker in colorectal cancer
|Han SX,Wang J,Wang LJ,Jin GH,Ying X,He CC,Guo XJ,Zhang JY,Zhang Y,Zhu Q||Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (35:6149)||2014|