Invitrogen

RCBTB2 Polyclonal Antibody

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RCBTB2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemichal analysis of RCBTB2 in PC-3 cells using a Polyclonal antibody (Product # PA5-112684) at a dilution of 1:100. A Cy3-congugated Goat Anti-Rabbit IgG secondary antibody was used.

RCBTB2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunocytochemical analysis of paraffin-embeded RCBTB2 in human lung cancer using a RCBTB2 Polyclonal antibody (Product # PA5-112684) at a dilution of 1:100.

RCBTB2 Antibody in Western Blot (WB) — Western Blot analysis of RCBTB2 in multiple sample types using a RCBTB2 polyclonal antibody (Product # PA5-112684) at a concentration of 3.2 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: HepG2 whole cell lysate, Rat liver tissue, Rat brain tissue, Rat stomach tissue .

Product Details

PA5-112684

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:5,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
ELISA (ELISA)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human RCC1 and BTB domain-containing protein 2 protein (1-200AA) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	4 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.03% ProClin 300
Storage conditions	-20°C or -80°C if preferred
RRID	AB_2867419

Target Information

RCBTB2 is a member of the RCC1-related GEF family. The N-terminal half of the amino acid sequence shows similarity to the regulator of chromosome condensation RCC1, which acts as a guanine nucleotide exchange factor (GEF) protein for the Ras-related GTPase Ran.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CHC1-L; Chromosome condensation 1-like; RCC1 and BTB domain-containing protein 2; RCC1-like G exchanging factor; RCC1-like G exchanging factor RLG; regulator of chromosome condensation (RCC1) and BTB (POZ) domain containing protein 2; Regulator of chromosome condensation and BTB domain-containing protein 2

Gene Aliases: CHC1L; RCBTB2; RLG

UniProt ID: (Human) O95199, (Rat) Q6P798

Entrez Gene ID: (Human) 1102, (Rat) 290363

Molecular Function: guanyl-nucleotide exchange factor