Proteintech

RCC1 Polyclonal Antibody

RCC1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 17

RCC1 Antibody (22142-1-AP) in ICC/IF

Immunofluorescent analysis of A431 cells using 22142-1-AP (RCC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

RCC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RCC1 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

RCC1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

22142-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RCC1 Fusion Protein Ag17569 (72-421 aa encoded by BC010067)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.38 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1.

Immunogen sequence: AEAGGMHTV CLSKSGQVYS FGCNDEGALG RDTSVEGSEM VPGKVELQEK VVQVSAGDSH TAALTDDGRV FLWGSFRDNN GVIGLLEPMK KSMVPVQVQL DVPVVKVASG NDHLVMLTAD GDLYTLGCGE QGQLGRVPEL FANRGGRQGL ERLLVPKCVM LKSRGSRGHV RFQDAFCGAY FTFAISHEGH VYGFGLSNYH QLGTPGTESC FIPQNLTSFK NSTKSWVGFS GGQHHTVCMD SEGKAYSLGR AEYGRLGLGE GAEEKSIPTL ISRLPAVSSV ACGASVGYAV TKDGRVFAWG MGTNYQLGTG QDEDAWSPVE MMGKQLENRV VLSVSSGGQH TVLLVKDKEQ S (72-421 aa encoded by BC010067)

Target Information

Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.

Experimental gerontology

Iyer SR,Hsia RC,Folker ES,Lovering RM

Thu Jul 15 00:00:00 EDT 2021

Selective Peptidomimetic Inhibitors of NTMT1/2: Rational Design, Synthesis, Characterization, and Crystallographic Studies.

Journal of medicinal chemistry

Mackie BD,Chen D,Dong G,Dong C,Parker H,Schaner Tooley CE,Noinaj N,Min J,Huang R

Thu Sep 10 00:00:00 EDT 2020

Differences in Mitotic Spindle Architecture in Mammalian Neural Stem Cells Influence Mitotic Accuracy during Brain Development.

Current biology : CB

Vargas-Hurtado D,Brault JB,Piolot T,Leconte L,Da Silva N,Pennetier C,Baffet A,Marthiens V,Basto R

Mon Sep 23 00:00:00 EDT 2019

Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.

Biomaterials

Song Y,Tang C,Yin C

Sat Dec 01 00:00:00 EST 2018

Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.

Biomaterials

Song Y,Tang C,Yin C

Sat Dec 01 00:00:00 EST 2018

Regulator of chromatin condensation 1 abrogates the G1 cell cycle checkpoint via Cdk1 in human papillomavirus E7-expressing epithelium and cervical cancer cells.

Cell death & disease

Qiao L,Zheng J,Tian Y,Zhang Q,Wang X,Chen JJ,Zhang W

Tue May 22 00:00:00 EDT 2018

Regulator of chromatin condensation 1 abrogates the G1 cell cycle checkpoint via Cdk1 in human papillomavirus E7-expressing epithelium and cervical cancer cells.

Cell death & disease

Qiao L,Zheng J,Tian Y,Zhang Q,Wang X,Chen JJ,Zhang W

Tue May 22 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Cell cycle regulatory protein; Chromosome condensation protein 1; guanine nucleotide-releasing protein; Regulator of chromosome condensation; SNHG3-RCC1 readthrough

View more View less

Gene Aliases: 4931417M11Rik; AI326872; CHC1; RCC1; RCC1-I; SNHG3-RCC1

View more View less

UniProt ID: (Human) P18754, (Mouse) Q8VE37

View more View less

Entrez Gene ID: (Human) 1104, (Mouse) 100088, (Rat) 682908

View more View less

Function(s)

chromatin binding Ran guanyl-nucleotide exchange factor activity protein binding nucleosomal DNA binding histone binding DNA binding guanyl-nucleotide exchange factor activity

Process(es)

G1/S transition of mitotic cell cycle cell cycle mitotic spindle organization chromosome segregation mitotic nuclear division regulation of mitotic nuclear division positive regulation of GTPase activity spindle assembly cell division viral process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

RCC1 Polyclonal Antibody

RCC1 Antibody (22142-1-AP) in ICC/IF

Product Details

22142-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!