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RCL1 Antibody (PA5-110284) in WB
Western blot analysis of RCL1 in extracts of various cell lines. Samples were incubated with RCL1 Polyclonal antibody (Product # PA5-110284) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-110284
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human RCL1 (NP_005763.3).
Conjugate
Form
Liquid
Concentration
3.27 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855695
Product Specific Information
Immunogen sequence: MATQAHSLSY AGCNFLRQRL VLSTLSGRPV KIRKIRARDD NPGLRDFEAS FIRLLDKITN GSRIEINQTG TTLYYQPGLL YGGSVEHDCS VLRGIGYYLE SLLCLAPFMK HPLKIVLRGV TNDQVDPSVD VLKATALPLL KQFGIDGESF ELKIVRRGMP PGGGGEVVFS CPVRKVLKPI QLTDPGKIKR IRGMAYSVRV SPQMANRIVD SARSILNKFI PDIYIYTDHM KGVNSGKSPG FGLSLVAETT SGTFLSAELA SNPQGQGAAV LPEDLGRNCA RLLLEEIYRG GCVDSTNQSL
Target Information
RCL1, which does not have cyclase activity, plays a role in 40S- ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: RNA 3'-terminal phosphate cyclase-like protein; RNA cyclase homolog; RNA terminal phosphate cyclase-like 1
Gene Aliases: 2310040A02Rik; Ab1-353; Ac1292; AI789745; C76567; HSPC338; RCL1; RNAC; RPC2; RPCL1; RTC2
UniProt ID: (Human) Q9Y2P8, (Mouse) Q9JJT0
Entrez Gene ID: (Human) 10171, (Mouse) 59028, (Rat) 309301
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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