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Agrisera
RDR2/RNA-dependent RNA polymerase 2 Polyclonal Antibody
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FIGURE: 1 / 1
RDR2/RNA-dependent RNA polymerase 2 Antibody (AS15-3097) in WB
50 µg of total protein from Arabidopsis thaliana: rdr 2-1 mutant, T-DNA insertion first exon (a), 30 µg protein from wild-type Col-0 (b), 50 µg protein from wild-type Col-0 (c), extracted with extraction buffer (50 m M Tris p H 7.5; 150 m M Na Cl; 1 m M EDTA; 10% v/v Glycerin; 1 m M DTT, 1 x Complete Protease Inhibitor Cocktail, Roche) and denatured with LaemmLi buffer at 95°C/5 min., were separated on 7.5%... View More
Product Details
AS15-3097
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana RDR2 sequence, located towards C-terminal part of the protein.
Conjugate
Form
Lyophilized
Amount
200 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
RDR2 encodes a nuclear RNA-dependent RNA polymerase that functions in the RNA-directed DNA methylation pathway by converting plant-specific RNA polymerase IV transcripts from repetitive, heterochromatic regions (including many transposable elements) into double-stranded RNA that is subsequently processed into predominantly 24-nt small interfering RNAs to guide transcriptional silencing and genome stability. The gene is protein-coding and produces an RDR2 protein that localizes to the nucleus and is also observed in nucleolar-associated siRNA processing centers together with other pathway components. Structurally, the locus comprises 4 exons and encodes a large, eukaryotic-type RNA-dependent RNA polymerase containing a conserved catalytic RdRP core domain; biochemical and structural work indicates RDR2 engages RNA templates (including RNA within RNA/DNA hybrids under defined end-structure constraints) and initiates complementary-strand synthesis near template 3' ends, consistent with tight functional coupling to polymerase IV transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AtRDR2; RNA-dependent RNA polymerase 2; SILENCING MOVEMENT DEFICIENT 1; SMD1; T22B4.110; T22B4_110
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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