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Agrisera
RDR6/RNA-dependent RNA polymerase 6 Polyclonal Antibody
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FIGURE: 1 / 1
RDR6/RNA-dependent RNA polymerase 6 Antibody (AS15-3098) in WB
50 µg of total protein from Arabidopsis thaliana whole vegetative rosette wild type Col-0(a)rdr 6-12 mutant (b)extracted with extraction buffer (50 m M Tris p H 7.5; 150 m M Na Cl; 1 m M EDTA; 10% v/v Glycerin; 1 m M DTT, 1 x Complete Protease Inhibitor Cocktail, Roche) and denatured with LaemmLi buffer at 95°C/5 min., were separated on 7.5% SDS-PAGE and blotted 1.5 h to PVDF using tank transfer. Blots were... View More
Product Details
AS15-3098
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana RDR6 sequence.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
RDR6 encodes a cytoplasmic RNA-dependent RNA polymerase that functions in plant RNA silencing, acting downstream of small-RNA targeting to convert single-stranded RNA templates into double-stranded RNA that is subsequently processed into secondary siRNAs to reinforce and spread post-transcriptional gene silencing, including trans-acting siRNA biogenesis and antiviral defense. The encoded protein is a eukaryotic-type RdRP with a conserved catalytic core characteristic of RNA polymerases, including the essential Asp residue required for polymerase activity, and it also exhibits terminal nucleotidyltransferase activity and primer-independent as well as primer-dependent initiation on RNA templates in vitro. Structurally, RDR6 is a large, multi-domain protein containing an N-terminal RNA recognition motif-like region and a C-terminal RdRP domain that supports dsRNA synthesis needed for amplification (transitivity) of silencing signals and robust maintenance of silencing across tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AtRDR6; SDE1; SGS2; SILENCING DEFECTIVE 1; SUPPRESSOR OF GENE SILENCING 2
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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