Immunofluorescent analysis of RED showing staining in the nucleus of A549 cells. A549 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a RED polyclonal antibody (Product # PA5-32151) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 251 and 480 of Human RED|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-32151 targets RED in WB applications and shows reactivity with Human samples.
The PA5-32151 immunogen is recombinant protein fragment corresponding to a region within amino acids 251 and 480 of Human RED.
The protein encoded by this gene was identified by its RED repeat, a stretch of repeated arginine, glutamic acid and aspartic acid residues. The protein localizes to discrete dots within the nucleus, excluding the nucleolus. Its function is unknown. This gene maps to chromosome 5; however, a pseudogene may exist on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.