Invitrogen

RELM alpha Polyclonal Antibody, PeproTech®

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Antibody Testing Data (3)

RELM alpha Antibody in Western Blot (WB)

FIGURE: 1 / 3

RELM alpha Antibody (500-P214-1MG) in WB

Western Blot: To detect Murine RELMalpha by Western Blot analysis RELM alpha Polyclonal Antibody (Product # 500-P214-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine RELMalpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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Product Details

500-P214-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 10 publications 10 publications

ELISA (ELISA)

0.5-2.0 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

In vitro Assay (IV)

View 3 publications 3 publications

Immunostaining (IS)

View 12 publications 12 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine RELMalpha

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929850

Product Specific Information

AA Sequence of recombinant protein: MDETIEIIVE NKVKELLANP ANYPSTVTKT LSCTSVKTMN RWASCPAGMT ATGCACGFAC GSWEIQSGDT CNCLCLLVDW TTARCCQLS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine RELMalpha. Anti-Murine RELMalpha-specific antibody was purified by affinity chromatography employing an immobilized Murine RELMalpha matrix.

Sandwich ELISA: To detect Murine RELMalpha by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine RELMalpha (500-P214Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine RELMalpha.

Western Blot: To detect Murine RELMalpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine RELMalpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

RELM alpha, also known as Resistin-like alpha or FIZZ1, is a small cytokine (88 aminoacids, MW 9.4 kDa) produced mostly by macrophages. Its highest constitutive expression has been observed in small peritoneal macrophages, alveolar macrophages and adipose tissue macrophages. It is strongly induced by IL-4 and IL-13. Together with Arginase 1 and Ym-1, RELM alpha is considered one of the hallmarks of the M2 polarization of mouse macrophages, however, macrophages polarized with IL-10 (M2c) express neither RELM alpha nor Arginase 1. Also, the expression patterns of RELM alpha and Arginase 1 in untreated tissue macrophages significantly differ. Elevated levels of RELM alpha may indicate parasitic infection. Humans do not have RELM alpha gene but human Resistin resembles mouse RELM alpha in respect to sequence, tissue distribution and function. Other members of RELM family in mouse: RELM beta, RELM gamma and Resistin display different tissue distribution and functional properties. RELM alpha plays an important role in parasite infection, mucosal inflammation and allergy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Group-2 innate lymphoid cell-dependent regulation of tissue neutrophil migration by alternatively activated macrophage-secreted Ear11.

Mucosal immunology

Panova V,Gogoi M,Rodriguez-Rodriguez N,Sivasubramaniam M,Jolin HE,Heycock MWD,Walker JA,Rana BMJ,Drynan LF,Hodskinson M,Pannell R,King G,Wing M,Easton AJ,Oedekoven CA,Kent DG,Fallon PG,Barlow JL,McKenzie ANJ

Fri Jan 01 00:00:00 EST 2021

Differential sensitivity of inflammatory macrophages and alternatively activated macrophages to ferroptosis.

European journal of immunology

Piattini F,Matsushita M,Muri J,Bretscher P,Feng X,Freigang S,Dalli J,Schneider C,Kopf M

500-P214 was used in Flow cytometry/Cell sorting to suggest that activation cues of tissue macrophages determine sensitivity to lipid peroxidation and ferroptotic cell death.

Fri Oct 01 00:00:00 EDT 2021

SREBP1-induced fatty acid synthesis depletes macrophages antioxidant defences to promote their alternative activation.

Nature metabolism

Bidault G,Virtue S,Petkevicius K,Jolin HE,Dugourd A,Guénantin AC,Leggat J,Mahler-Araujo B,Lam BYH,Ma MK,Dale M,Carobbio S,Kaser A,Fallon PG,Saez-Rodriguez J,McKenzie ANJ,Vidal-Puig A

500-P214 was used in Flow cytometry/Cell sorting to investigate the effects of SREBP1 on macrophage alternative activation.

Wed Sep 01 00:00:00 EDT 2021

SREBP1-induced fatty acid synthesis depletes macrophages antioxidant defences to promote their alternative activation.

Nature metabolism

Bidault G,Virtue S,Petkevicius K,Jolin HE,Dugourd A,Guénantin AC,Leggat J,Mahler-Araujo B,Lam BYH,Ma MK,Dale M,Carobbio S,Kaser A,Fallon PG,Saez-Rodriguez J,McKenzie ANJ,Vidal-Puig A

500-P214 was used in Flow cytometry/Cell sorting to investigate the effects of SREBP1 on macrophage alternative activation.

Wed Sep 01 00:00:00 EDT 2021

IL-17A both initiates, via IFNγ suppression, and limits the pulmonary type-2 immune response to nematode infection.

Mucosal immunology

Ajendra J,Chenery AL,Parkinson JE,Chan BHK,Pearson S,Colombo SAP,Boon L,Grencis RK,Sutherland TE,Allen JE

Sun Nov 01 00:00:00 EDT 2020

BHLHE40 Promotes T_H2 Cell-Mediated Antihelminth Immunity and Reveals Cooperative CSF2RB Family Cytokines.

Journal of immunology (Baltimore, Md. : 1950)

Jarjour NN,Bradstreet TR,Schwarzkopf EA,Cook ME,Lai CW,Huang SC,Taneja R,Stappenbeck TS,Van Dyken SJ,Urban JF,Edelson BT

Sat Feb 15 00:00:00 EST 2020

Select autophagy genes maintain quiescence of tissue-resident macrophages and increase susceptibility to Listeria monocytogenes.

Nature microbiology

Wang YT,Zaitsev K,Lu Q,Li S,Schaiff WT,Kim KW,Droit L,Wilen CB,Desai C,Balce DR,Orchard RC,Orvedahl A,Park S,Kreamalmeyer D,Handley SA,Pfeifer JD,Baldridge MT,Artyomov MN,Stallings CL,Virgin HW

500-P214 was used in Flow cytometry/Cell sorting to delineate a function of the autophagy proteins Beclin 1 and FIP200-but not of other essential autophagy components ATG5, ATG16L1 or ATG7-in mediating quiescence of tissue-resident macrophages by limiting the effects of systemic interferon-γ.

Sat Feb 01 00:00:00 EST 2020

A Macrophage-Pericyte Axis Directs Tissue Restoration via Amphiregulin-Induced Transforming Growth Factor Beta Activation.

Immunity

Minutti CM,Modak RV,Macdonald F,Li F,Smyth DJ,Dorward DA,Blair N,Husovsky C,Muir A,Giampazolias E,Dobie R,Maizels RM,Kendall TJ,Griggs DW,Kopf M,Henderson NC,Zaiss DM

Tue Mar 19 00:00:00 EDT 2019

Inflammasome-Independent Role for NLRP3 in Controlling Innate Antihelminth Immunity and Tissue Repair in the Lung.

Journal of immunology (Baltimore, Md. : 1950)

Chenery AL,Alhallaf R,Agha Z,Ajendra J,Parkinson JE,Cooper MM,Chan BHK,Eichenberger RM,Dent LA,Robertson AAB,Kupz A,Brough D,Loukas A,Sutherland TE,Allen JE,Giacomin PR

Fri Nov 15 00:00:00 EST 2019

The major secreted protein of the whipworm parasite tethers to matrix and inhibits interleukin-13 function.

Nature communications

Bancroft AJ,Levy CW,Jowitt TA,Hayes KS,Thompson S,Mckenzie EA,Ball MD,Dubaissi E,France AP,Bellina B,Sharpe C,Mironov A,Brown SL,Cook PC,S MacDonald A,Thornton DJ,Grencis RK

Tue May 28 00:00:00 EDT 2019

Mitochondrial Membrane Potential Regulates Nuclear Gene Expression in Macrophages Exposed to Prostaglandin E2.

Immunity

Sanin DE,Matsushita M,Klein Geltink RI,Grzes KM,van Teijlingen Bakker N,Corrado M,Kabat AM,Buck MD,Qiu J,Lawless SJ,Cameron AM,Villa M,Baixauli F,Patterson AE,Hässler F,Curtis JD,O'Neill CM,O'Sullivan D,Wu D,Mittler G,Huang SC,Pearce EL,Pearce EJ

Tue Dec 18 00:00:00 EST 2018

Tissue-Restricted Adaptive Type 2 Immunity Is Orchestrated by Expression of the Costimulatory Molecule OX40L on Group 2 Innate Lymphoid Cells.

Immunity

Halim TYF,Rana BMJ,Walker JA,Kerscher B,Knolle MD,Jolin HE,Serrao EM,Haim-Vilmovsky L,Teichmann SA,Rodewald HR,Botto M,Vyse TJ,Fallon PG,Li Z,Withers DR,McKenzie ANJ

Tue Jun 19 00:00:00 EDT 2018

Migratory CD103+ dendritic cells suppress helminth-driven type 2 immunity through constitutive expression of IL-12.

The Journal of experimental medicine

Everts B,Tussiwand R,Dreesen L,Fairfax KC,Huang SC,Smith AM,O'Neill CM,Lam WY,Edelson BT,Urban JF,Murphy KM,Pearce EJ

Mon Jan 11 00:00:00 EST 2016

NADPH Oxidases Are Essential for Macrophage Differentiation.

The Journal of biological chemistry

Xu Q,Choksi S,Qu J,Jang J,Choe M,Banfi B,Engelhardt JF,Liu ZG

Fri Sep 16 00:00:00 EDT 2016

Pathophysiological mechanisms underlying phenotypic differences in pulmonary radioresponse.

Scientific reports

Jackson IL,Zhang Y,Bentzen SM,Hu J,Zhang A,Vujaskovic Z

500-P214 was used in Immunohistochemistry to investigate radiation-induced lung injury response in mouse models.

Tue Nov 15 00:00:00 EST 2016

Pathophysiological mechanisms underlying phenotypic differences in pulmonary radioresponse.

Scientific reports

Jackson IL,Zhang Y,Bentzen SM,Hu J,Zhang A,Vujaskovic Z

500-P214 was used in Immunohistochemistry to investigate radiation-induced lung injury response in mouse models.

Tue Nov 15 00:00:00 EST 2016

Pathophysiological mechanisms underlying phenotypic differences in pulmonary radioresponse.

Scientific reports

Jackson IL,Zhang Y,Bentzen SM,Hu J,Zhang A,Vujaskovic Z

500-P214 was used in Immunohistochemistry to investigate radiation-induced lung injury response in mouse models.

Tue Nov 15 00:00:00 EST 2016

Dynamic changes in macrophage activation and proliferation during the development and resolution of intestinal inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Little MC,Hurst RJ,Else KJ

Sat Nov 01 00:00:00 EDT 2014

Dynamic changes in macrophage activation and proliferation during the development and resolution of intestinal inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Little MC,Hurst RJ,Else KJ

Sat Nov 01 00:00:00 EDT 2014

M2-like macrophages are responsible for collagen degradation through a mannose receptor-mediated pathway.

The Journal of cell biology

Madsen DH,Leonard D,Masedunskas A,Moyer A,Jürgensen HJ,Peters DE,Amornphimoltham P,Selvaraj A,Yamada SS,Brenner DA,Burgdorf S,Engelholm LH,Behrendt N,Holmbeck K,Weigert R,Bugge TH

Mon Sep 16 00:00:00 EDT 2013

Group V secretory phospholipase A2 is involved in macrophage activation and is sufficient for macrophage effector functions in allergic pulmonary inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Ohta S,Imamura M,Xing W,Boyce JA,Balestrieri B

Sat Jun 15 00:00:00 EDT 2013

Group V secretory phospholipase A2 is involved in macrophage activation and is sufficient for macrophage effector functions in allergic pulmonary inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Ohta S,Imamura M,Xing W,Boyce JA,Balestrieri B

Sat Jun 15 00:00:00 EDT 2013

Alternaria induces STAT6-dependent acute airway eosinophilia and epithelial FIZZ1 expression that promotes airway fibrosis and epithelial thickness.

Journal of immunology (Baltimore, Md. : 1950)

Doherty TA,Khorram N,Sugimoto K,Sheppard D,Rosenthal P,Cho JY,Pham A,Miller M,Croft M,Broide DH

Thu Mar 15 00:00:00 EDT 2012

Resistin-like molecule α1 (Fizz1) recruits lung dendritic cells without causing pulmonary fibrosis.

Respiratory research

Madala SK,Edukulla R,Davis KR,Schmidt S,Davidson C,Kitzmiller JA,Hardie WD,Korfhagen TR

Fri Jun 22 00:00:00 EDT 2012

Impaired wound healing with defective expression of chemokines and recruitment of myeloid cells in TLR3-deficient mice.

Journal of immunology (Baltimore, Md. : 1950)

Lin Q,Fang D,Fang J,Ren X,Yang X,Wen F,Su SB

Tue Mar 15 00:00:00 EDT 2011

Human gingiva-derived mesenchymal stem cells elicit polarization of m2 macrophages and enhance cutaneous wound healing.

Stem cells (Dayton, Ohio)

Zhang QZ,Su WR,Shi SH,Wilder-Smith P,Xiang AP,Wong A,Nguyen AL,Kwon CW,Le AD

Fri Oct 01 00:00:00 EDT 2010

Differential roles of macrophages in diverse phases of skin repair.

Journal of immunology (Baltimore, Md. : 1950)

Lucas T,Waisman A,Ranjan R,Roes J,Krieg T,Müller W,Roers A,Eming SA

Thu Apr 01 00:00:00 EDT 2010

Alternatively activated macrophage-derived RELM-{alpha} is a negative regulator of type 2 inflammation in the lung.

The Journal of experimental medicine

Nair MG,Du Y,Perrigoue JG,Zaph C,Taylor JJ,Goldschmidt M,Swain GP,Yancopoulos GD,Valenzuela DM,Murphy A,Karow M,Stevens S,Pearce EJ,Artis D

Mon Apr 13 00:00:00 EDT 2009

Differential impact of L-arginine deprivation on the activation and effector functions of T cells and macrophages.

Journal of leukocyte biology

Choi BS,Martinez-Falero IC,Corset C,Munder M,Modolell M,Müller I,Kropf P

Sun Feb 01 00:00:00 EST 2009

Resistin-like molecule alpha decreases glucose tolerance during intestinal inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Munitz A,Seidu L,Cole ET,Ahrens R,Hogan SP,Rothenberg ME

Sun Feb 15 00:00:00 EST 2009

Plasmodium chabaudi limits early Nippostrongylus brasiliensis-induced pulmonary immune activation and Th2 polarization in co-infected mice.

BMC immunology

Hoeve MA,Mylonas KJ,Fairlie-Clarke KJ,Mahajan SM,Allen JE,Graham AL

Tue Dec 01 00:00:00 EST 2009

IL-31-IL-31R interactions negatively regulate type 2 inflammation in the lung.

The Journal of experimental medicine

Perrigoue JG,Li J,Zaph C,Goldschmidt M,Scott P,de Sauvage FJ,Pearce EJ,Ghilardi N,Artis D

Mon Mar 19 00:00:00 EDT 2007

Novel effector molecules in type 2 inflammation: lessons drawn from helminth infection and allergy.

Journal of immunology (Baltimore, Md. : 1950)

Nair MG,Guild KJ,Artis D

Tue Aug 01 00:00:00 EDT 2006

Bioinformatics

Protein Aliases: Cysteine-rich secreted protein A12-gamma; Cysteine-rich secreted protein FIZZ1; FIZZ2; found in inflammatory zone 1; HXCP2; Hypoxia-induced mitogenic factor; Parasite-induced macrophage novel gene 1 protein; RELM-beta; RELMalpha; RELMb; Resistin-like alpha

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Gene Aliases: 1810019L16Rik; Fizz-1; Fizz1; HIMF; Pmng1; RELM-alpha; RELMa; RELMalpha; Retnla; Xcp2

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UniProt ID: (Mouse) Q9D8Y4

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Entrez Gene ID: (Mouse) 57262

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Function(s)

hormone activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RELM alpha Polyclonal Antibody, PeproTech®

RELM alpha Antibody (500-P214-1MG) in WB

Product Details

500-P214-1MG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

In vitro Assay (IV)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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