|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues V(31) E N K V K E L L A N P A N(44).|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4257 detects the RELM alpha protein in mouse and human samples.
PA1-4257 has been successfully used in Western blot procedures. By Western blot this antibody detects a ~15 kDa protein corresponding to secreted mouse RELM alpha from the media of transfected 293T cells.
The PA1-4257 immunizing peptide corresponds to amino acid residues 31-44 of the mouse RELM alpha protein.
A family of resistin-like molecules (RELMs) has been identified in rodents and humans. Resistin is a hormone produced by fat cells. RELM alpha is a secreted protein that has a restricted tissue distribution with highest levels in adipose tissue. Another family member, RELM beta, is a secreted protein expressed only in the gastrointestinal tract, particularly the colon, in both mouse and human. RELM beta gene expression is highest in proliferative epithelial cells and is markedly increased in tumors, suggesting a role in intestinal proliferation. Resistin and the RELMs share a cysteine composition and other signature features. Thus, the RELMs together with resistin comprise a class of tissue-specific signaling molecules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.